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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 73(3-4)

FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION

https://doi.org/10.18999/nagjms.73.3-4.129
https://doi.org/10.18999/nagjms.73.3-4.129
1944e590-380f-486c-8df3-020617423716
名前 / ファイル ライセンス アクション
v73n34p129_135.pdf v73n34p129_135.pdf (333.9 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2011-08-24
タイトル
タイトル FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION
著者 KIMURA, MACHIKO

× KIMURA, MACHIKO

WEKO 42179

KIMURA, MACHIKO

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HARA, MASAAKI

× HARA, MASAAKI

WEKO 42180

HARA, MASAAKI

Search repository
ITAKURA, ATSUO

× ITAKURA, ATSUO

WEKO 42181

ITAKURA, ATSUO

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SATO, CHIAKI

× SATO, CHIAKI

WEKO 42182

SATO, CHIAKI

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IKEBUCHI, KENJI

× IKEBUCHI, KENJI

WEKO 42183

IKEBUCHI, KENJI

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ISHIHARA, OSAMU

× ISHIHARA, OSAMU

WEKO 42184

ISHIHARA, OSAMU

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キーワード
主題Scheme Other
主題 Free fetal DNA
キーワード
主題Scheme Other
主題 Prenatal diagnosis
キーワード
主題Scheme Other
主題 Electrophoresis
キーワード
主題Scheme Other
主題 Size fractionation
抄録
内容記述 Free fetal DNA (ffDNA) in maternal plasma has now become a valuable source for noninvasive prenatal diagnosis. Being able to accurately identify the size of ffDNA in maternal plasma is essential for a noninvasive prenatal diagnosis. Furthermore, it is important to investigate the molecular characteristics related to apoptosis which gives rise to ffDNA. We investigated the fragment size of ffDNA in each sample more precisely, using both Y-STR and SRY primers, in 20 maternal plasma samples from the 17th to 39th weeks of gestation. PCR was conducted with Y-STR and SRY primers which can be used to amplify 100–524 bp fragments. In samples from 10 pregnant women carrying male fetuses, the maximum fragment size detected by Y-STR and SRY primers ranged from 219 to 313 bp. As a result, the mean average maximum fragment size of free fetal DNA detected by Y-STR and SRY primers was 286±28 bp. The Y-STR alleles detected in each maternal plasma DNA sample were all in agreement with the results of their cord blood samples. We concluded that the fragment size of ffDNA comprises 2 nucleosomal complexes or less, but not exceeding 3.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.73.3-4.129
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 73, 号 3-4, p. 129-135, 発行日 2011-08
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7334/7334.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/15355
識別子タイプ HDL
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