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FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION
https://doi.org/10.18999/nagjms.73.3-4.129
https://doi.org/10.18999/nagjms.73.3-4.1291944e590-380f-486c-8df3-020617423716
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v73n34p129_135.pdf (333.9 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2011-08-24 | |||||
| タイトル | ||||||
| タイトル | FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION | |||||
| 言語 | en | |||||
| 著者 |
KIMURA, MACHIKO
× KIMURA, MACHIKO× HARA, MASAAKI× ITAKURA, ATSUO× SATO, CHIAKI× IKEBUCHI, KENJI× ISHIHARA, OSAMU |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Free fetal DNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Prenatal diagnosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Electrophoresis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Size fractionation | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Free fetal DNA (ffDNA) in maternal plasma has now become a valuable source for noninvasive prenatal diagnosis. Being able to accurately identify the size of ffDNA in maternal plasma is essential for a noninvasive prenatal diagnosis. Furthermore, it is important to investigate the molecular characteristics related to apoptosis which gives rise to ffDNA. We investigated the fragment size of ffDNA in each sample more precisely, using both Y-STR and SRY primers, in 20 maternal plasma samples from the 17th to 39th weeks of gestation. PCR was conducted with Y-STR and SRY primers which can be used to amplify 100–524 bp fragments. In samples from 10 pregnant women carrying male fetuses, the maximum fragment size detected by Y-STR and SRY primers ranged from 219 to 313 bp. As a result, the mean average maximum fragment size of free fetal DNA detected by Y-STR and SRY primers was 286±28 bp. The Y-STR alleles detected in each maternal plasma DNA sample were all in agreement with the results of their cord blood samples. We concluded that the fragment size of ffDNA comprises 2 nucleosomal complexes or less, but not exceeding 3. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.73.3-4.129 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7334/7334.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 73, 号 3-4, p. 129-135, 発行日 2011-08 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/15355 | |||||
| 識別子タイプ | HDL | |||||
