{"_buckets": {"deposit": "57baba0f-e9fc-4014-9867-82b18ed5ad88"}, "_deposit": {"id": "13456", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13456"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00013456"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3-4", "bibliographicPageEnd": "135", "bibliographicPageStart": "129", "bibliographicVolumeNumber": "73", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Free fetal DNA (ffDNA) in maternal plasma has now become a valuable source for noninvasive prenatal diagnosis. Being able to accurately identify the size of ffDNA in maternal plasma is essential for a noninvasive prenatal diagnosis. Furthermore, it is important to investigate the molecular characteristics related to apoptosis which gives rise to ffDNA. We investigated the fragment size of ffDNA in each sample more precisely, using both Y-STR and SRY primers, in 20 maternal plasma samples from the 17th to 39th weeks of gestation. PCR was conducted with Y-STR and SRY primers which can be used to amplify 100\u2013524 bp fragments. In samples from 10 pregnant women carrying male fetuses, the maximum fragment size detected by Y-STR and SRY primers ranged from 219 to 313 bp. As a result, the mean average maximum fragment size of free fetal DNA detected by Y-STR and SRY primers was 286\u00b128 bp. The Y-STR alleles detected in each maternal plasma DNA sample were all in agreement with the results of their cord blood samples. We concluded that the fragment size of ffDNA comprises 2 nucleosomal complexes or less, but not exceeding 3.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7334/7334.html"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/15355"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.73.3-4.129", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KIMURA, MACHIKO"}], "nameIdentifiers": [{"nameIdentifier": "42179", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HARA, MASAAKI"}], "nameIdentifiers": [{"nameIdentifier": "42180", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ITAKURA, ATSUO"}], "nameIdentifiers": [{"nameIdentifier": "42181", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SATO, CHIAKI"}], "nameIdentifiers": [{"nameIdentifier": "42182", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IKEBUCHI, KENJI"}], "nameIdentifiers": [{"nameIdentifier": "42183", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ISHIHARA, OSAMU"}], "nameIdentifiers": [{"nameIdentifier": "42184", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v73n34p129_135.pdf", "filesize": [{"value": "333.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 333900.0, "url": {"label": "v73n34p129_135.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/13456/files/v73n34p129_135.pdf"}, "version_id": "7c3fab62-fe35-470b-bc56-93b103869a73"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Free fetal DNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prenatal diagnosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Electrophoresis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Size fractionation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/1359"], "permalink_uri": "https://doi.org/10.18999/nagjms.73.3-4.129", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2011-08-24"}, "publish_date": "2011-08-24", "publish_status": "0", "recid": "13456", "relation": {}, "relation_version_is_last": true, "title": ["FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION"], "weko_shared_id": null}