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Involvement of reduced sensitivity to Ca2+ in b-adrenergic action on airway smooth muscle
http://hdl.handle.net/2237/2750
http://hdl.handle.net/2237/2750db5bb854-59a5-4f8a-890e-fd7c7883c6d0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Oguma_paper_050118.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-27 | |||||
| タイトル | ||||||
| タイトル | Involvement of reduced sensitivity to Ca2+ in b-adrenergic action on airway smooth muscle | |||||
| 言語 | en | |||||
| その他のタイトル | ||||||
| その他のタイトル | Loss of Ca2+ sensitization in b-adrenergic action | |||||
| 言語 | en | |||||
| 著者 |
Oguma, Tetsuya
× Oguma, Tetsuya× Kume, Hiroaki× Ito, Satoru× Takeda, Naoya× Honjo, Haruo× Kodama, Itsuo× Shimokata, Kaoru× Kamiya, Kaichiro× 神谷, 香一郎 |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | b-adrenergic receptor agonists | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ca2+ sensitization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | myosin phosphatase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gs | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Rho | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To determine the involvement of Ca2+ sensitization in b-adrenergic action, we examined the relationship between isometric tension and intracellular Ca2+ concentration (F340/F380) in the inhibitory action of isoproterenol (ISO) against methacholine (MCh)- induced contraction, using fura-2 loaded tracheal smooth muscle.\nISO reduces contraction more than Ca2+ concentration. This phenomenon was mimicked by forskolin and db-cAMP. In contrast, an inhibition in tension by SKF- 96365, a non-selective Ca2+ channel inhibitor, was associated with that in F340/F380, different from ISO. In the presence of Rp-cAMP, a membrane-permeable inhibitor of protein kinase A (PKA), ISO caused an equivalent relaxation with a less reduction in F340/F380. The effects of ISO were not affected in the presence of Y-27632, an inhibitor of Rho-kinase, and bisindolylmaleimide, an inhibitor of protein kinase C. Even removal of Ca2+ from the extracellular surface, the effects of ISO was not affected. ISO also inhibits high K+-induced contraction without lowering F340/F380. However, response to ISO was completely diminished in the presence of calyculin A, an inhibitor of myosin phosphatase. In conclusion, b-adrenergic action antagonizes Ca2+ sensitization mediated by impairment of myosin phosphatase activity. Moreover, cAMP independent process, G protein direct action, is more potent in inhibiting this Ca2+ sensitization than PKA. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Blackwell Scientific Publication | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/j.1365-2222.2006.02412.x | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 09547894 | |||||
| 書誌情報 |
en : Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 巻 36, 号 2, p. 183-191, 発行日 2006-02 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/2750 | |||||
| 識別子タイプ | HDL | |||||
