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  1. F100 環境医学研究所
  2. F100b 紀要
  3. Environmental medicine : annual report of the Research Institute of Environmental Medicine
  4. 46(1-2)

Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD

https://doi.org/10.18999/envm.46.36
https://doi.org/10.18999/envm.46.36
02e4b79a-215b-4566-8009-395127c0acef
名前 / ファイル ライセンス アクション
KJ00000034857.pdf KJ00000034857.pdf (640.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2006-01-05
タイトル
タイトル Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD
言語 en
著者 OHMORI, Sachiko

× OHMORI, Sachiko

WEKO 3096

en OHMORI, Sachiko

Search repository
HAYASHI, Yoshitaka

× HAYASHI, Yoshitaka

WEKO 3097

en HAYASHI, Yoshitaka

Search repository
YAMAMOTO, Michiyo

× YAMAMOTO, Michiyo

WEKO 3098

en YAMAMOTO, Michiyo

Search repository
KAMBE, Fukushi

× KAMBE, Fukushi

WEKO 3099

en KAMBE, Fukushi

Search repository
OGAWA, Masamichi

× OGAWA, Masamichi

WEKO 3100

en OGAWA, Masamichi

Search repository
KAMIJO, Takashi

× KAMIJO, Takashi

WEKO 3101

en KAMIJO, Takashi

Search repository
SEO, Hisao

× SEO, Hisao

WEKO 3102

en SEO, Hisao

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 transgenic mice
キーワード
主題Scheme Other
主題 growth hormone (GH)
キーワード
主題Scheme Other
主題 type II IGHD
抄録
内容記述 We identified several mutations in the intron 3 of human growth hormone gene I (hGH-I) in patients with isolated GH deficiency (IGHD) type II characterized by an autosomal dominant trait. The mutations result in exon 3 skipping and generation of 17 Kd mutant GH. To elucidate how the mutation causes dominant trait, transgenic mice expressing a mutant hGH gene (the first guanine to adenine transversion in intron 3: GH-I; IVS3+1: G-A) were produced in C57BL/6 strain. Genotypes of mice were identified by PCR-amplified products of tail snip DNAs. Delivery of the mutant hGH transgene into 76 fertilized eggs resulted in production of two male heterozygous transgenic mice (hGH^<+/->, the zero filial generation, FO). Since the mating of the transgenic mice with the same strain was unsuccessful, they were outcrossed with CD-I (ICR) strain. Only one mouse gave birth, producing 4 male and 7 female (F1) harboring the mutant hGH gene in one allele (hGH^<+/->). F1 mice were mated again with the wild type ICR strain, generating 82 hGH+/" mice (F2 : 51 males and 31 females). To study whether somatotrophs in F2 mice express the mutant hGH gene, RNA extracted from the pituitary was subjected to RT-PCR. It was demonstrated that the F2, hGH^<+/-> mice express the mutant hGH gene, lacking exon 3. Thus, these heterozygous mice were sib-mated to generate homozygous mice (F3). The mating resulted in 27% hGH^<-/->, 64% hGH+/ and 9% hGH^<+/+> mice, indicating that the transgene was carried stably to the descendants and did not interfere with the reproduction. These mice will be a valuable model to study how type II IGHD develops during the course of development.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 国立情報学研究所で電子化したコンテンツを使用している。
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Research Institute of Environmental Medicine, Nagoya University
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.18999/envm.46.36
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 02870517
書誌情報 en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University

巻 46, 号 1/2, p. 36-39, 発行日 2002-12
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/2777
識別子タイプ HDL
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