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Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD
https://doi.org/10.18999/envm.46.36
https://doi.org/10.18999/envm.46.3602e4b79a-215b-4566-8009-395127c0acef
名前 / ファイル | ライセンス | アクション |
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KJ00000034857.pdf (640.2 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-01-05 | |||||
タイトル | ||||||
タイトル | Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD | |||||
言語 | en | |||||
著者 |
OHMORI, Sachiko
× OHMORI, Sachiko× HAYASHI, Yoshitaka× YAMAMOTO, Michiyo× KAMBE, Fukushi× OGAWA, Masamichi× KAMIJO, Takashi× SEO, Hisao |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transgenic mice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | growth hormone (GH) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | type II IGHD | |||||
抄録 | ||||||
内容記述 | We identified several mutations in the intron 3 of human growth hormone gene I (hGH-I) in patients with isolated GH deficiency (IGHD) type II characterized by an autosomal dominant trait. The mutations result in exon 3 skipping and generation of 17 Kd mutant GH. To elucidate how the mutation causes dominant trait, transgenic mice expressing a mutant hGH gene (the first guanine to adenine transversion in intron 3: GH-I; IVS3+1: G-A) were produced in C57BL/6 strain. Genotypes of mice were identified by PCR-amplified products of tail snip DNAs. Delivery of the mutant hGH transgene into 76 fertilized eggs resulted in production of two male heterozygous transgenic mice (hGH^<+/->, the zero filial generation, FO). Since the mating of the transgenic mice with the same strain was unsuccessful, they were outcrossed with CD-I (ICR) strain. Only one mouse gave birth, producing 4 male and 7 female (F1) harboring the mutant hGH gene in one allele (hGH^<+/->). F1 mice were mated again with the wild type ICR strain, generating 82 hGH+/" mice (F2 : 51 males and 31 females). To study whether somatotrophs in F2 mice express the mutant hGH gene, RNA extracted from the pituitary was subjected to RT-PCR. It was demonstrated that the F2, hGH^<+/-> mice express the mutant hGH gene, lacking exon 3. Thus, these heterozygous mice were sib-mated to generate homozygous mice (F3). The mating resulted in 27% hGH^<-/->, 64% hGH+/ and 9% hGH^<+/+> mice, indicating that the transgene was carried stably to the descendants and did not interfere with the reproduction. These mice will be a valuable model to study how type II IGHD develops during the course of development. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 国立情報学研究所で電子化したコンテンツを使用している。 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Research Institute of Environmental Medicine, Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/envm.46.36 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 02870517 | |||||
書誌情報 |
en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 巻 46, 号 1/2, p. 36-39, 発行日 2002-12 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/2777 | |||||
識別子タイプ | HDL |