{"_buckets": {"deposit": "41748f5c-30aa-4485-a668-b8b074cd8855"}, "_deposit": {"id": "1367", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1367"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00001367"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1/2", "bibliographicPageEnd": "39", "bibliographicPageStart": "36", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We identified several mutations in the intron 3 of human growth hormone gene I (hGH-I) in patients with isolated GH deficiency (IGHD) type II characterized by an autosomal dominant trait. The mutations result in exon 3 skipping and generation of 17 Kd mutant GH. To elucidate how the mutation causes dominant trait, transgenic mice expressing a mutant hGH gene (the first guanine to adenine transversion in intron 3: GH-I; IVS3+1: G-A) were produced in C57BL/6 strain. Genotypes of mice were identified by PCR-amplified products of tail snip DNAs. Delivery of the mutant hGH transgene into 76 fertilized eggs resulted in production of two male heterozygous transgenic mice (hGH^\u003c+/-\u003e, the zero filial generation, FO). Since the mating of the transgenic mice with the same strain was unsuccessful, they were outcrossed with CD-I (ICR) strain. Only one mouse gave birth, producing 4 male and 7 female (F1) harboring the mutant hGH gene in one allele (hGH^\u003c+/-\u003e). F1 mice were mated again with the wild type ICR strain, generating 82 hGH+/\" mice (F2 : 51 males and 31 females). To study whether somatotrophs in F2 mice express the mutant hGH gene, RNA extracted from the pituitary was subjected to RT-PCR. It was demonstrated that the F2, hGH^\u003c+/-\u003e mice express the mutant hGH gene, lacking exon 3. Thus, these heterozygous mice were sib-mated to generate homozygous mice (F3). The mating resulted in 27% hGH^\u003c-/-\u003e, 64% hGH+/ and 9% hGH^\u003c+/+\u003e mice, indicating that the transgene was carried stably to the descendants and did not interfere with the reproduction. These mice will be a valuable model to study how type II IGHD develops during the course of development.", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u56fd\u7acb\u60c5\u5831\u5b66\u7814\u7a76\u6240\u3067\u96fb\u5b50\u5316\u3057\u305f\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3002", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/2777"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/envm.46.36", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Research Institute of Environmental Medicine, Nagoya University"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "02870517", "subitem_source_identifier_type": "ISSN"}]}, "item_9_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OHMORI, Sachiko"}], "nameIdentifiers": [{"nameIdentifier": "3096", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HAYASHI, Yoshitaka"}], "nameIdentifiers": [{"nameIdentifier": "3097", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMAMOTO, Michiyo"}], "nameIdentifiers": [{"nameIdentifier": "3098", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAMBE, Fukushi"}], "nameIdentifiers": [{"nameIdentifier": "3099", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OGAWA, Masamichi"}], "nameIdentifiers": [{"nameIdentifier": "3100", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAMIJO, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "3101", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SEO, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "3102", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000034857.pdf", "filesize": [{"value": "640.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 640200.0, "url": {"label": "KJ00000034857.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/1367/files/KJ00000034857.pdf"}, "version_id": "bf04ea3a-0f5a-4fc0-bc3a-1f5ded2c6abf"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "transgenic mice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "growth hormone (GH)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "type II IGHD", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD"}]}, "item_type_id": "9", "owner": "1", "path": ["329/333/334/335"], "permalink_uri": "https://doi.org/10.18999/envm.46.36", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2006-01-05"}, "publish_date": "2006-01-05", "publish_status": "0", "recid": "1367", "relation": {}, "relation_version_is_last": true, "title": ["Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD"], "weko_shared_id": null}