ログイン
言語:

WEKO3

  • トップ
  • コミュニティ
  • ランキング
AND
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "41748f5c-30aa-4485-a668-b8b074cd8855"}, "_deposit": {"id": "1367", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1367"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00001367"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1/2", "bibliographicPageEnd": "39", "bibliographicPageStart": "36", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We identified several mutations in the intron 3 of human growth hormone gene I (hGH-I) in patients with isolated GH deficiency (IGHD) type II characterized by an autosomal dominant trait. The mutations result in exon 3 skipping and generation of 17 Kd mutant GH. To elucidate how the mutation causes dominant trait, transgenic mice expressing a mutant hGH gene (the first guanine to adenine transversion in intron 3: GH-I; IVS3+1: G-A) were produced in C57BL/6 strain. Genotypes of mice were identified by PCR-amplified products of tail snip DNAs. Delivery of the mutant hGH transgene into 76 fertilized eggs resulted in production of two male heterozygous transgenic mice (hGH^\u003c+/-\u003e, the zero filial generation, FO). Since the mating of the transgenic mice with the same strain was unsuccessful, they were outcrossed with CD-I (ICR) strain. Only one mouse gave birth, producing 4 male and 7 female (F1) harboring the mutant hGH gene in one allele (hGH^\u003c+/-\u003e). F1 mice were mated again with the wild type ICR strain, generating 82 hGH+/\" mice (F2 : 51 males and 31 females). To study whether somatotrophs in F2 mice express the mutant hGH gene, RNA extracted from the pituitary was subjected to RT-PCR. It was demonstrated that the F2, hGH^\u003c+/-\u003e mice express the mutant hGH gene, lacking exon 3. Thus, these heterozygous mice were sib-mated to generate homozygous mice (F3). The mating resulted in 27% hGH^\u003c-/-\u003e, 64% hGH+/ and 9% hGH^\u003c+/+\u003e mice, indicating that the transgene was carried stably to the descendants and did not interfere with the reproduction. These mice will be a valuable model to study how type II IGHD develops during the course of development.", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u56fd\u7acb\u60c5\u5831\u5b66\u7814\u7a76\u6240\u3067\u96fb\u5b50\u5316\u3057\u305f\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3002", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/2777"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/envm.46.36", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Research Institute of Environmental Medicine, Nagoya University"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "02870517", "subitem_source_identifier_type": "ISSN"}]}, "item_9_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OHMORI, Sachiko"}], "nameIdentifiers": [{"nameIdentifier": "3096", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HAYASHI, Yoshitaka"}], "nameIdentifiers": [{"nameIdentifier": "3097", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMAMOTO, Michiyo"}], "nameIdentifiers": [{"nameIdentifier": "3098", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAMBE, Fukushi"}], "nameIdentifiers": [{"nameIdentifier": "3099", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OGAWA, Masamichi"}], "nameIdentifiers": [{"nameIdentifier": "3100", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAMIJO, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "3101", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SEO, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "3102", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000034857.pdf", "filesize": [{"value": "640.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 640200.0, "url": {"label": "KJ00000034857.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/1367/files/KJ00000034857.pdf"}, "version_id": "bf04ea3a-0f5a-4fc0-bc3a-1f5ded2c6abf"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "transgenic mice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "growth hormone (GH)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "type II IGHD", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD"}]}, "item_type_id": "9", "owner": "1", "path": ["329/333/334/335"], "permalink_uri": "https://doi.org/10.18999/envm.46.36", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2006-01-05"}, "publish_date": "2006-01-05", "publish_status": "0", "recid": "1367", "relation": {}, "relation_version_is_last": true, "title": ["Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD"], "weko_shared_id": null}
  1. F100 環境医学研究所
  2. F100b 紀要
  3. Environmental medicine : annual report of the Research Institute of Environmental Medicine
  4. 46(1-2)

Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD

https://doi.org/10.18999/envm.46.36
02e4b79a-215b-4566-8009-395127c0acef
名前 / ファイル ライセンス アクション
KJ00000034857.pdf KJ00000034857.pdf (640.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2006-01-05
タイトル
タイトル Transgenic Mice Expressing A Mutant Human GH Gene Causing Type II IGHD
著者 OHMORI, Sachiko

× OHMORI, Sachiko

WEKO 3096

OHMORI, Sachiko

Search repository
HAYASHI, Yoshitaka

× HAYASHI, Yoshitaka

WEKO 3097

HAYASHI, Yoshitaka

Search repository
YAMAMOTO, Michiyo

× YAMAMOTO, Michiyo

WEKO 3098

YAMAMOTO, Michiyo

Search repository
KAMBE, Fukushi

× KAMBE, Fukushi

WEKO 3099

KAMBE, Fukushi

Search repository
OGAWA, Masamichi

× OGAWA, Masamichi

WEKO 3100

OGAWA, Masamichi

Search repository
KAMIJO, Takashi

× KAMIJO, Takashi

WEKO 3101

KAMIJO, Takashi

Search repository
SEO, Hisao

× SEO, Hisao

WEKO 3102

SEO, Hisao

Search repository
キーワード
主題Scheme Other
主題 transgenic mice
キーワード
主題Scheme Other
主題 growth hormone (GH)
キーワード
主題Scheme Other
主題 type II IGHD
抄録
内容記述 We identified several mutations in the intron 3 of human growth hormone gene I (hGH-I) in patients with isolated GH deficiency (IGHD) type II characterized by an autosomal dominant trait. The mutations result in exon 3 skipping and generation of 17 Kd mutant GH. To elucidate how the mutation causes dominant trait, transgenic mice expressing a mutant hGH gene (the first guanine to adenine transversion in intron 3: GH-I; IVS3+1: G-A) were produced in C57BL/6 strain. Genotypes of mice were identified by PCR-amplified products of tail snip DNAs. Delivery of the mutant hGH transgene into 76 fertilized eggs resulted in production of two male heterozygous transgenic mice (hGH^<+/->, the zero filial generation, FO). Since the mating of the transgenic mice with the same strain was unsuccessful, they were outcrossed with CD-I (ICR) strain. Only one mouse gave birth, producing 4 male and 7 female (F1) harboring the mutant hGH gene in one allele (hGH^<+/->). F1 mice were mated again with the wild type ICR strain, generating 82 hGH+/" mice (F2 : 51 males and 31 females). To study whether somatotrophs in F2 mice express the mutant hGH gene, RNA extracted from the pituitary was subjected to RT-PCR. It was demonstrated that the F2, hGH^<+/-> mice express the mutant hGH gene, lacking exon 3. Thus, these heterozygous mice were sib-mated to generate homozygous mice (F3). The mating resulted in 27% hGH^<-/->, 64% hGH+/ and 9% hGH^<+/+> mice, indicating that the transgene was carried stably to the descendants and did not interfere with the reproduction. These mice will be a valuable model to study how type II IGHD develops during the course of development.
内容記述タイプ Abstract
内容記述
内容記述 国立情報学研究所で電子化したコンテンツを使用している。
内容記述タイプ Other
出版者
出版者 Research Institute of Environmental Medicine, Nagoya University
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/envm.46.36
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 02870517
書誌情報 Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University

巻 46, 号 1/2, p. 36-39, 発行日 2002-12
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/2777
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 21:38:31.364000
Show All versions

Share

Mendeley CiteULike Twitter Facebook Print Addthis

Cite as

Export

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by CERN Data Centre & Invenio


Powered by CERN Data Centre & Invenio