{"created":"2021-03-01T06:07:43.579668+00:00","id":1368,"links":{},"metadata":{"_buckets":{"deposit":"3be1e1b0-193f-4d73-bc2f-652ca2cc64b9"},"_deposit":{"id":"1368","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1368"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00001368","sets":["329:333:334:335"]},"author_link":["3103","3104","3105"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2","bibliographicPageEnd":"42","bibliographicPageStart":"40","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We identified ZAKI-4 as a thyroid hormone (T3) responsive gene in human skin fibroblasts. Our recent study revealed that two ZAKI-4 isoforms, α and β, are generated from a single gene on chromosome 6. Only expression of α is increased by T3, while that of β is not affected. Both isoforms belong to a family of proteins containing a conserved motif, which interacts with calcineurin and inhibits its activity. Recently, expression of a mouse homologue of the ZAKI-4 family gene (MCIP1) is shown to be induced by the activation of calcineurin in myocytes. We thus studied whether ZAKI-4 expression is also regulated by calcineurin-mediated pathway. Northern blot analysis revealed activation of calcineurin by ionomycin/phorbol 12-myristate 13-acetate (PMA) did not increase the expression of both isoforms. In addition, either basal expression of the two isoforms or T3-induced ZAKI-4α expression was not affected by FK506, a specific calcineurin inhibitor, suggesting the activation of calcineurin is not involved in ZAKI-4 regulation. However, cyclosporin A (CsA), another inhibitor of calcineurin decreased the basal expression of ZAKI-4α. Furthermore, T3-mediated increase of ZAKI-4α was completely blocked by CsA, but not by FK506. It is thus indicated that the two isoforms are differentially regulated, and the regulation of ZAKI-4α involves a CsA-sensitive pathway, which is independent of calcineurin activation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"国立情報学研究所で電子化したコンテンツを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/2778"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.46.40","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"02870517","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"CAO, Xia","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3103","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KAMBE, Fukushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3104","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SEO, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3105","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"KJ00000034858.pdf","filesize":[{"value":"549.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000034858.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/1368/files/KJ00000034858.pdf"},"version_id":"bd3939d0-18d7-401d-a334-fc6444c94cdf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ZAKI-4","subitem_subject_scheme":"Other"},{"subitem_subject":"calcineurin","subitem_subject_scheme":"Other"},{"subitem_subject":"CsA","subitem_subject_scheme":"Other"},{"subitem_subject":"FK506","subitem_subject_scheme":"Other"},{"subitem_subject":"thyroid hormone","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-01-05"},"publish_date":"2006-01-05","publish_status":"0","recid":"1368","relation_version_is_last":true,"title":["Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-26T05:57:16.218031+00:00"}