@article{oai:nagoya.repo.nii.ac.jp:00001372,
 author = {MITSUYAMA, Hirohito and KAMBE, Fukushi and MURAKAMI, Ryuichiro and ISHIGURO, Naoki and SEO, Hisao},
 issue = {1/2},
 journal = {Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University},
 month = {Dec},
 note = {We previously reported that an increase in intracellular calcium by calcium ionophore (A23187) and 4p-phorbol-12β- myristate-13α-acetate (PMA) induced the expression of IL-8 mRNA in human osteoblast-like HOS-TE85 cells and this induction was markedly suppressed by cyclosporin A (CsA). In this study we investigated whether the regulation by A23187, PMA and CsA was occured at a transcriptional level by reporter gene assays. A promoter region spanning from -1460 to +40 of IL-8 gene was PCR- amplified and inserted upstream of a luciferase reporter gene. Various deletion mutants were also constructed. Transfection of the plasmids into HOS-TE85 cells demonstrated that the nucleotides between -133 and -60 base pairs upstream of IL-8 gene are essential and sufficient for its induction by A23187/PMA, and suppression of this induction by CsA., 国立情報学研究所で電子化したコンテンツを使用している。},
 pages = {52--54},
 title = {Analysis of Interieukin-8 Gene Promoter function in Human Osteoblast-like Cells : Regulation by Ca^<2+>-signaling and Cyclosporin A},
 volume = {46},
 year = {2002}
}