{"created":"2021-03-01T06:07:43.831065+00:00","id":1372,"links":{},"metadata":{"_buckets":{"deposit":"faa9733a-5886-436e-bdf3-de9d62d2b80d"},"_deposit":{"id":"1372","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1372"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00001372","sets":["329:333:334:335"]},"author_link":["3124","3125","3126","3127","3128"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2","bibliographicPageEnd":"54","bibliographicPageStart":"52","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously reported that an increase in intracellular calcium by calcium ionophore (A23187) and 4p-phorbol-12β- myristate-13α-acetate (PMA) induced the expression of IL-8 mRNA in human osteoblast-like HOS-TE85 cells and this induction was markedly suppressed by cyclosporin A (CsA). In this study we investigated whether the regulation by A23187, PMA and CsA was occured at a transcriptional level by reporter gene assays. A promoter region spanning from -1460 to +40 of IL-8 gene was PCR- amplified and inserted upstream of a luciferase reporter gene. Various deletion mutants were also constructed. Transfection of the plasmids into HOS-TE85 cells demonstrated that the nucleotides between -133 and -60 base pairs upstream of IL-8 gene are essential and sufficient for its induction by A23187/PMA, and suppression of this induction by CsA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"国立情報学研究所で電子化したコンテンツを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/2782"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.46.52","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"02870517","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MITSUYAMA, Hirohito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3124","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KAMBE, Fukushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3125","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MURAKAMI, Ryuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3126","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISHIGURO, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3127","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SEO, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3128","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"KJ00000034862.pdf","filesize":[{"value":"352.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000034862.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/1372/files/KJ00000034862.pdf"},"version_id":"a232e35b-954d-4466-ae00-c949c0e2cfda"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"interleukin-8","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclosporin A","subitem_subject_scheme":"Other"},{"subitem_subject":"human osteoblast","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analysis of Interieukin-8 Gene Promoter function in Human Osteoblast-like Cells : Regulation by Ca^<2+>-signaling and Cyclosporin A","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of Interieukin-8 Gene Promoter function in Human Osteoblast-like Cells : Regulation by Ca^<2+>-signaling and Cyclosporin A","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-01-05"},"publish_date":"2006-01-05","publish_status":"0","recid":"1372","relation_version_is_last":true,"title":["Analysis of Interieukin-8 Gene Promoter function in Human Osteoblast-like Cells : Regulation by Ca^<2+>-signaling and Cyclosporin A"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-26T05:58:38.903766+00:00"}