@article{oai:nagoya.repo.nii.ac.jp:00001373,
 author = {KANOU, Yasuhiko and ABE, Naoki and ISHIDA, Junji and FUKAMIZU, Akiyoshi and SEO, Hisao and MURATA, Yoshiharu},
 issue = {1/2},
 journal = {Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University},
 month = {Dec},
 note = {ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice., 国立情報学研究所で電子化したコンテンツを使用している。},
 pages = {55--57},
 title = {Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression},
 volume = {46},
 year = {2002}
}