{"created":"2021-03-01T06:07:43.894180+00:00","id":1373,"links":{},"metadata":{"_buckets":{"deposit":"6d4f5b47-c996-4cad-bec9-bc4bf86e2ea1"},"_deposit":{"id":"1373","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1373"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00001373","sets":["329:333:334:335"]},"author_link":["3129","3130","3131","3132","3133","3134"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2","bibliographicPageEnd":"57","bibliographicPageStart":"55","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"国立情報学研究所で電子化したコンテンツを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/2783"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.46.55","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"02870517","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KANOU, Yasuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3129","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ABE, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3130","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISHIDA, Junji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3131","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"FUKAMIZU, Akiyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3132","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SEO, Hisao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3133","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MURATA, Yoshiharu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3134","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"KJ00000034863.pdf","filesize":[{"value":"430.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000034863.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/1373/files/KJ00000034863.pdf"},"version_id":"e86aca93-3818-42da-b86b-06b8f58802b5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ZAKI-4","subitem_subject_scheme":"Other"},{"subitem_subject":"homologous recombination","subitem_subject_scheme":"Other"},{"subitem_subject":"gene targeting","subitem_subject_scheme":"Other"},{"subitem_subject":"calcineurin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-01-05"},"publish_date":"2006-01-05","publish_status":"0","recid":"1373","relation_version_is_last":true,"title":["Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-26T05:58:38.946437+00:00"}