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Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C
https://doi.org/10.18999/envm.46.81
https://doi.org/10.18999/envm.46.8112a6cb59-b444-4951-9ab1-14edfae68e70
名前 / ファイル | ライセンス | アクション |
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KJ00000034871.pdf (246.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-01-05 | |||||
タイトル | ||||||
タイトル | Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C | |||||
言語 | en | |||||
著者 |
ZHANG, Liyan
× ZHANG, Liyan× LEE, Jong-Kook× KODAMA, Itsuo |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | G protein-activated inwardly rectifying K channels | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein kinase C | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phosphorylation site | |||||
抄録 | ||||||
内容記述 | G protein-activated inwardly rectifying K^+ channels (GIRK) are inhibited by activation of protein kinase C (PKC), but the exact mechanisms are not known. GIRK channels in the heart are heterotetrama composed of GIRK1 and GIRK4 subunits. We investigated the phosphorylation sites by site-directed mutagenesis procedure in GIRK4 subunits heterologously expressed in Xenopus oocytes. Homomeric wild type (WT) GIRK4 current amplitude was inhibited by phorbor-1,2 myrisitate-acetate (PMA: a PKC activator), by 45.5±1.8% (n=5). Serine or theorine at five potential phosphorylation sites of GIRK4 channel were replaced by alanine to prepare five different single mutants and a penta mutant with replacement of all the sites. PMA (1 |0,M) application to these mutant GIRK4 channels resulted in a similar reduction of current amplitude (by 45-62%) as observed in WT channels (by 45.5±1.8%). From these observations, it is suggested that an inhibition of GIRK4 channels by PKC activation is not mediated by direct phosphorylation of the channel protein. Instead, some indirect PKC actions on other molecules may be involved. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 国立情報学研究所で電子化したコンテンツを使用している。 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Research Institute of Environmental Medicine, Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/envm.46.81 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 02870517 | |||||
書誌情報 |
en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 巻 46, 号 1/2, p. 81-83, 発行日 2002-12 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/2791 | |||||
識別子タイプ | HDL |