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  1. F100 環境医学研究所
  2. F100b 紀要
  3. Environmental medicine : annual report of the Research Institute of Environmental Medicine
  4. 46(1-2)

Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C

https://doi.org/10.18999/envm.46.81
https://doi.org/10.18999/envm.46.81
12a6cb59-b444-4951-9ab1-14edfae68e70
名前 / ファイル ライセンス アクション
KJ00000034871.pdf KJ00000034871.pdf (246.3 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2006-01-05
タイトル
タイトル Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C
言語 en
著者 ZHANG, Liyan

× ZHANG, Liyan

WEKO 3166

en ZHANG, Liyan

Search repository
LEE, Jong-Kook

× LEE, Jong-Kook

WEKO 3167

en LEE, Jong-Kook

Search repository
KODAMA, Itsuo

× KODAMA, Itsuo

WEKO 3168

en KODAMA, Itsuo

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 G protein-activated inwardly rectifying K channels
キーワード
主題Scheme Other
主題 protein kinase C
キーワード
主題Scheme Other
主題 phosphorylation site
抄録
内容記述 G protein-activated inwardly rectifying K^+ channels (GIRK) are inhibited by activation of protein kinase C (PKC), but the exact mechanisms are not known. GIRK channels in the heart are heterotetrama composed of GIRK1 and GIRK4 subunits. We investigated the phosphorylation sites by site-directed mutagenesis procedure in GIRK4 subunits heterologously expressed in Xenopus oocytes. Homomeric wild type (WT) GIRK4 current amplitude was inhibited by phorbor-1,2 myrisitate-acetate (PMA: a PKC activator), by 45.5±1.8% (n=5). Serine or theorine at five potential phosphorylation sites of GIRK4 channel were replaced by alanine to prepare five different single mutants and a penta mutant with replacement of all the sites. PMA (1 |0,M) application to these mutant GIRK4 channels resulted in a similar reduction of current amplitude (by 45-62%) as observed in WT channels (by 45.5±1.8%). From these observations, it is suggested that an inhibition of GIRK4 channels by PKC activation is not mediated by direct phosphorylation of the channel protein. Instead, some indirect PKC actions on other molecules may be involved.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 国立情報学研究所で電子化したコンテンツを使用している。
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Research Institute of Environmental Medicine, Nagoya University
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.18999/envm.46.81
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 02870517
書誌情報 en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University

巻 46, 号 1/2, p. 81-83, 発行日 2002-12
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/2791
識別子タイプ HDL
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