{"created":"2021-03-01T06:07:44.537560+00:00","id":1381,"links":{},"metadata":{"_buckets":{"deposit":"efde1a2c-e728-49f5-96b3-b655e80b76b6"},"_deposit":{"id":"1381","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1381"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00001381","sets":["329:333:334:335"]},"author_link":["3166","3167","3168"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2","bibliographicPageEnd":"83","bibliographicPageStart":"81","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"G protein-activated inwardly rectifying K^+ channels (GIRK) are inhibited by activation of protein kinase C (PKC), but the exact mechanisms are not known. GIRK channels in the heart are heterotetrama composed of GIRK1 and GIRK4 subunits. We investigated the phosphorylation sites by site-directed mutagenesis procedure in GIRK4 subunits heterologously expressed in Xenopus oocytes. Homomeric wild type (WT) GIRK4 current amplitude was inhibited by phorbor-1,2 myrisitate-acetate (PMA: a PKC activator), by 45.5±1.8% (n=5). Serine or theorine at five potential phosphorylation sites of GIRK4 channel were replaced by alanine to prepare five different single mutants and a penta mutant with replacement of all the sites. PMA (1 |0,M) application to these mutant GIRK4 channels resulted in a similar reduction of current amplitude (by 45-62%) as observed in WT channels (by 45.5±1.8%). From these observations, it is suggested that an inhibition of GIRK4 channels by PKC activation is not mediated by direct phosphorylation of the channel protein. Instead, some indirect PKC actions on other molecules may be involved.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"国立情報学研究所で電子化したコンテンツを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/2791"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.46.81","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"02870517","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ZHANG, Liyan","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"LEE, Jong-Kook","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3167","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KODAMA, Itsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3168","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"KJ00000034871.pdf","filesize":[{"value":"246.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000034871.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/1381/files/KJ00000034871.pdf"},"version_id":"69dfa6c6-941a-480a-97c3-eb688affdfe6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"G protein-activated inwardly rectifying K channels","subitem_subject_scheme":"Other"},{"subitem_subject":"protein kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphorylation site","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-01-05"},"publish_date":"2006-01-05","publish_status":"0","recid":"1381","relation_version_is_last":true,"title":["Molecular Mechanisms for Regulation of the G Protein-activated Inwardly Rectifying K^+ (GIRK) Channels by Protein Kinase C"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-26T05:58:47.983485+00:00"}