{"created":"2021-03-01T06:07:44.788352+00:00","id":1385,"links":{},"metadata":{"_buckets":{"deposit":"410e706d-06bc-4696-aab5-30143649ced0"},"_deposit":{"id":"1385","owners":[],"pid":{"revision_id":0,"type":"depid","value":"1385"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00001385","sets":["329:333:334:335"]},"author_link":["3185","3186","3187","3188","3189","3190"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2","bibliographicPageEnd":"94","bibliographicPageStart":"92","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pluripotent embryonic stem (ES) cells differentiate to cardiac myocytes in vitro by many other previous reports demonstrated \"hanging-drop\" method. In this study, the number of ES cells in each hanging-drop plays an important role in the cultivation of cardiac myocytes. We examined the optimal hanging-drop size to obtain embryonic stem cell-derived cardiac cells (ESCMs) in vitro using specific labeled mouse ES cells (hCGP7) which were stably transfected with the enhanced green fluorescent protein (GFP) under transcriptional control of the cardiac myocyte-specific promoter with fluorescence-activated cell sorting (FACS) analysis. GFP-positive cells were sorted efficiently in the hanging-drop cell number of approximately 800～1600 cells/drop. The present data will be an important element to establish the optimal methods of ES cellular differentiation in vitro.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"国立情報学研究所で電子化したコンテンツを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/2795"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.46.92","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"02870517","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIWA, Keiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3185","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"LEE, Jong-Kook","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3186","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HIDAKA, Kyoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3187","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SHI, Rong-qian","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3188","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MORISAKI, Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3189","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KODAMA, Itsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3190","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-16"}],"displaytype":"detail","filename":"KJ00000034875.pdf","filesize":[{"value":"272.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000034875.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/1385/files/KJ00000034875.pdf"},"version_id":"d0fc76fd-afc7-4577-8def-38d8583d9489"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"embryonic stem cell","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence-activated cell sorting","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Optimal Population of Embryonic Stem Cells in \"Hanging Drop\" Culture for in-vitro Differentiation to Cardiac Myocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Optimal Population of Embryonic Stem Cells in \"Hanging Drop\" Culture for in-vitro Differentiation to Cardiac Myocytes","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-01-05"},"publish_date":"2006-01-05","publish_status":"0","recid":"1385","relation_version_is_last":true,"title":["Optimal Population of Embryonic Stem Cells in \"Hanging Drop\" Culture for in-vitro Differentiation to Cardiac Myocytes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-26T06:04:34.826956+00:00"}