@article{oai:nagoya.repo.nii.ac.jp:00014168,
 author = {YAMAMOTO, MASANORI and HIBI, HATSUKI and TSUJI, YOSHIKAZU and MIYAKE, KOJI},
 issue = {3-4},
 journal = {Nagoya Journal of Medical Science},
 month = {Dec},
 note = {The effects of protein synthesis inhibition and disassembly of microtubules in the epididymal epithelia on proluminal movement of 3H-androgens were investigated by using in vivo microperifusion of 3H-testosterone and subsequent micropuncture to obtain peritubular and intraluminal fluids of caput epididymal tubules. Cycloheximide (100 μg/ml) was used as protein synthesis inhibitor. Nocodazole (3 μg/ml) was used to depolymerize microtubules in the cell. The perifusion fluid was Minimum Essential Medium containing 26.7 μCi/ml 3H-testosterone and 1.3 μCi/ml 14C-polyethyleneglycol (14C-PEG), or the same fluid supplemented with cycloheximide or nocodazole. Radioactivity of 3H-androgen and 14C-PEG in perifusion and intraluminal fluids was determined at one hour after initiation of the sustaining perifusion, and the percentage of radioactivity of 3H-androgen and 14C-PEG appearing in the intraluminal fluid to that in the peritubular fluid was determined. Proluminal movement of 3H-androgens into the caput epididymal tubules in the control rats was 323.4±73.2%. This value was significantly reduced to 121.8±13% by addition of cycloheximide to the perifusion fluid (p<O.O1). Transepithelial movement of 3H-androgen in the caput epididymis was significantly decreased to 86.6±5.3% by exposure of the epididymal tubules to nocodazole (p<O.O1). Inhibition of protein synthesis and disassembly of microtubules in the epididymal epithelial cells completely eliminated antigrade proluminal movement of 3H-androgen into the tubules. Study of the incorporation of 35S-Methionine into epididymal tissue protein revealed significant reduction of the quantity of radiolabeled proteins in the perifused tissue with fluid containing cycloheximide (p<O.O1). These results suggest that antigrade proluminal androgen movement may be related to the synthesis of intracellular cytoplasmic proteins.},
 pages = {119--126},
 title = {EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS},
 volume = {57},
 year = {1994}
}