{"created":"2021-03-01T06:21:14.972279+00:00","id":14168,"links":{},"metadata":{"_buckets":{"deposit":"c7ff55df-d88b-435f-a841-49a1070f9c27"},"_deposit":{"created_by":17,"id":"14168","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"14168"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00014168","sets":["499:508:509:614"]},"author_link":["98926","98927","98928","98929"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-12-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"126","bibliographicPageStart":"119","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The effects of protein synthesis inhibition and disassembly of microtubules in the epididymal epithelia on proluminal movement of 3H-androgens were investigated by using in vivo microperifusion of 3H-testosterone and subsequent micropuncture to obtain peritubular and intraluminal fluids of caput epididymal tubules. Cycloheximide (100 μg/ml) was used as protein synthesis inhibitor. Nocodazole (3 μg/ml) was used to depolymerize microtubules in the cell. The perifusion fluid was Minimum Essential Medium containing 26.7 μCi/ml 3H-testosterone and 1.3 μCi/ml 14C-polyethyleneglycol (14C-PEG), or the same fluid supplemented with cycloheximide or nocodazole. Radioactivity of 3H-androgen and 14C-PEG in perifusion and intraluminal fluids was determined at one hour after initiation of the sustaining perifusion, and the percentage of radioactivity of 3H-androgen and 14C-PEG appearing in the intraluminal fluid to that in the peritubular fluid was determined. Proluminal movement of 3H-androgens into the caput epididymal tubules in the control rats was 323.4±73.2%. This value was significantly reduced to 121.8±13% by addition of cycloheximide to the perifusion fluid (p<O.O1). Transepithelial movement of 3H-androgen in the caput epididymis was significantly decreased to 86.6±5.3% by exposure of the epididymal tubules to nocodazole (p<O.O1). Inhibition of protein synthesis and disassembly of microtubules in the epididymal epithelial cells completely eliminated antigrade proluminal movement of 3H-androgen into the tubules. Study of the incorporation of 35S-Methionine into epididymal tissue protein revealed significant reduction of the quantity of radiolabeled proteins in the perifused tissue with fluid containing cycloheximide (p<O.O1). These results suggest that antigrade proluminal androgen movement may be related to the synthesis of intracellular cytoplasmic proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.57.3-4.119","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5734/5734.html","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMAMOTO, MASANORI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"98926","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HIBI, HATSUKI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"98927","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TSUJI, YOSHIKAZU","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"98928","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MIYAKE, KOJI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"98929","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"v57n34p119_126.pdf","filesize":[{"value":"557.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v57n34p119_126.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/14168/files/v57n34p119_126.pdf"},"version_id":"f31f5f1d-3c49-4de2-b971-37a0a4f99217"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Androgen","subitem_subject_scheme":"Other"},{"subitem_subject":"Epididymis","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytoplasmic receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"Methionine","subitem_subject_scheme":"Other"},{"subitem_subject":"Cycloheximide","subitem_subject_scheme":"Other"},{"subitem_subject":"Nocodazole","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["614"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-03-06"},"publish_date":"2012-03-06","publish_status":"0","recid":"14168","relation_version_is_last":true,"title":["EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T06:23:54.829026+00:00"}