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A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX
https://doi.org/10.18999/nagjms.59.1-2.37
https://doi.org/10.18999/nagjms.59.1-2.37cf38e71b-f2fb-4c3e-870c-b07ad004eaa3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v59n12p37_47.pdf (1.0 MB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-03-09 | |||||
| タイトル | ||||||
| タイトル | A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX | |||||
| 言語 | en | |||||
| 著者 |
MIZUTANI, HIDEKI
× MIZUTANI, HIDEKI× MERA, KAZUHIKO× UEDA, MINORU× IWATA, HISASHI |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bone morphogenetic protein (BMP) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bovine dentin matrix | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Purification | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.59.1-2.37 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 59, 号 1-2, p. 37-47, 発行日 1996-03-29 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/16100 | |||||
| 識別子タイプ | HDL | |||||
