{"_buckets": {"deposit": "1bf0bc1e-4cb9-4d7a-a265-33c8f31facb0"}, "_deposit": {"id": "14195", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "14195"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00014195"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-03-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "47", "bibliographicPageStart": "37", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/16100"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.59.1-2.37", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "MIZUTANI, HIDEKI"}], "nameIdentifiers": [{"nameIdentifier": "44196", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MERA, KAZUHIKO"}], "nameIdentifiers": [{"nameIdentifier": "44197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "UEDA, MINORU"}], "nameIdentifiers": [{"nameIdentifier": "44198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IWATA, HISASHI"}], "nameIdentifiers": [{"nameIdentifier": "44199", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v59n12p37_47.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "v59n12p37_47.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/14195/files/v59n12p37_47.pdf"}, "version_id": "42e9ab2b-7d82-46c9-bcc6-7a0c42e659e8"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Bone morphogenetic protein (BMP)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bovine dentin matrix", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Purification", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/617"], "permalink_uri": "https://doi.org/10.18999/nagjms.59.1-2.37", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-03-09"}, "publish_date": "2012-03-09", "publish_status": "0", "recid": "14195", "relation": {}, "relation_version_is_last": true, "title": ["A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX"], "weko_shared_id": null}