WEKO3
アイテム
{"_buckets": {"deposit": "1bf0bc1e-4cb9-4d7a-a265-33c8f31facb0"}, "_deposit": {"created_by": 17, "id": "14195", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "14195"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00014195", "sets": []}, "author_link": ["44196", "44197", "44198", "44199"], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-03-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "47", "bibliographicPageStart": "37", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/16100"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.59.1-2.37", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html", "subitem_relation_type_select": "URI"}}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN(Online)", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "EISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN(print)", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "MIZUTANI, HIDEKI", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44196", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MERA, KAZUHIKO", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "UEDA, MINORU", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IWATA, HISASHI", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44199", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v59n12p37_47.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "v59n12p37_47.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/14195/files/v59n12p37_47.pdf"}, "version_id": "42e9ab2b-7d82-46c9-bcc6-7a0c42e659e8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Bone morphogenetic protein (BMP)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bovine dentin matrix", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Purification", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "17", "path": ["617"], "permalink_uri": "https://doi.org/10.18999/nagjms.59.1-2.37", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-03-09"}, "publish_date": "2012-03-09", "publish_status": "0", "recid": "14195", "relation": {}, "relation_version_is_last": true, "title": ["A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX"], "weko_shared_id": -1}
A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX
https://doi.org/10.18999/nagjms.59.1-2.37
https://doi.org/10.18999/nagjms.59.1-2.37cf38e71b-f2fb-4c3e-870c-b07ad004eaa3
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-03-09 | |||||
タイトル | ||||||
タイトル | A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX | |||||
言語 | en | |||||
著者 |
MIZUTANI, HIDEKI
× MIZUTANI, HIDEKI× MERA, KAZUHIKO× UEDA, MINORU× IWATA, HISASHI |
|||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone morphogenetic protein (BMP) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bovine dentin matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Purification | |||||
抄録 | ||||||
内容記述 | We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.59.1-2.37 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 59, 号 1-2, p. 37-47, 発行日 1996-03-29 |
|||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/16100 | |||||
識別子タイプ | HDL |