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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 59(1-2)

A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX

https://doi.org/10.18999/nagjms.59.1-2.37
https://doi.org/10.18999/nagjms.59.1-2.37
cf38e71b-f2fb-4c3e-870c-b07ad004eaa3
名前 / ファイル ライセンス アクション
v59n12p37_47.pdf v59n12p37_47.pdf (1.0 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2012-03-09
タイトル
タイトル A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX
言語 en
著者 MIZUTANI, HIDEKI

× MIZUTANI, HIDEKI

WEKO 44196

en MIZUTANI, HIDEKI

Search repository
MERA, KAZUHIKO

× MERA, KAZUHIKO

WEKO 44197

en MERA, KAZUHIKO

Search repository
UEDA, MINORU

× UEDA, MINORU

WEKO 44198

en UEDA, MINORU

Search repository
IWATA, HISASHI

× IWATA, HISASHI

WEKO 44199

en IWATA, HISASHI

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 Bone morphogenetic protein (BMP)
キーワード
主題Scheme Other
主題 Bovine dentin matrix
キーワード
主題Scheme Other
主題 Purification
抄録
内容記述 We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.18999/nagjms.59.1-2.37
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ EISSN
収録物識別子 2186-3326
書誌情報 en : Nagoya Journal of Medical Science

巻 59, 号 1-2, p. 37-47, 発行日 1996-03-29
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/16100
識別子タイプ HDL
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