@article{oai:nagoya.repo.nii.ac.jp:00014195,
 author = {MIZUTANI, HIDEKI and MERA, KAZUHIKO and UEDA, MINORU and IWATA, HISASHI},
 issue = {1-2},
 journal = {Nagoya Journal of Medical Science},
 month = {Mar},
 note = {We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.},
 pages = {37--47},
 title = {A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX},
 volume = {59},
 year = {1996}
}