{"created":"2021-03-01T06:21:16.774392+00:00","id":14195,"links":{},"metadata":{"_buckets":{"deposit":"1bf0bc1e-4cb9-4d7a-a265-33c8f31facb0"},"_deposit":{"id":"14195","owners":[],"pid":{"revision_id":0,"type":"depid","value":"14195"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00014195"},"item_9_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"47","bibliographicPageStart":"37","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science"}]}]},"item_9_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5912/5912.html"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/16100"}]},"item_9_identifier_registration":{"attribute_name":"ID\u767b\u9332","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.59.1-2.37","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine"}]},"item_9_select_15":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN\uff08print\uff09","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIZUTANI, HIDEKI"}],"nameIdentifiers":[{"nameIdentifier":"44196","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MERA, KAZUHIKO"}],"nameIdentifiers":[{"nameIdentifier":"44197","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"UEDA, MINORU"}],"nameIdentifiers":[{"nameIdentifier":"44198","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IWATA, HISASHI"}],"nameIdentifiers":[{"nameIdentifier":"44199","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"v59n12p37_47.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v59n12p37_47.pdf","url":"https://nagoya.repo.nii.ac.jp/record/14195/files/v59n12p37_47.pdf"},"version_id":"42e9ab2b-7d82-46c9-bcc6-7a0c42e659e8"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"Bone morphogenetic protein (BMP)","subitem_subject_scheme":"Other"},{"subitem_subject":"Bovine dentin matrix","subitem_subject_scheme":"Other"},{"subitem_subject":"Purification","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX"}]},"item_type_id":"9","owner":"1","path":["499/508/509/617"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2012-03-09"},"publish_date":"2012-03-09","publish_status":"0","recid":"14195","relation_version_is_last":true,"title":["A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T18:12:47.329852+00:00"}