{"created":"2021-03-01T06:21:53.622759+00:00","id":14756,"links":{},"metadata":{"_buckets":{"deposit":"61eb9050-639b-40c7-a93c-d91d689a8f1b"},"_deposit":{"id":"14756","owners":[],"pid":{"revision_id":0,"type":"depid","value":"14756"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00014756","sets":["499:960:1165"]},"author_link":["45543","45544","45545","45546","45547","45548","45549","45550"],"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"229","bibliographicPageStart":"225","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Glycoconjugate Journal","bibliographic_titleLang":"en"}]}]},"item_12_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1997-02-01"}]},"item_12_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"名古屋大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"NAGOYA University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13901","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_12_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"}]},"item_12_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The human chorionic gonadotropin β-subunit (hCGβ) is a glycoprotein in which 12 cysteine residues pair to form six intramolecular disulfide bonds. In order to elucidate the effect of each disulfide bond on glycosylation of the molecule, we analysed structures of asparagine-linked oligosaccharides of various recombinant hCGβ produced in Chinese hamster ovary (CHO) cells: wild-type hCGβ (βWT) and mutants in which any one of the six intramolecular disulfide bonds had been disrupted by site-directed mutagenesis. SDS-PAGE analysis of βWT and these mutants before and after digestion with endoglycosidase F and H revealed structural changes in the oligosaccharide moieties of some mutants. In addition, structural analysis of oligosaccharides obtained from metabolically labeled βWT and a mutant showed that the mutant contained additional high mannose type oligosaccharides. These results suggest that elimination of a specific disulfide bond, resulting in a change in the protein conformation, disturbs the normal assembly of the mature complex type oligosaccharides in the hCGβ molecule. Abbreviations: hCGβ, human chorionic gonadotropin β-subunit; βWT, wild type hCGβ; CHO, Chinese hamster ovary; Endo-H, endoglycosidase H; Endo-F, endoglycosidase F","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_12_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"名古屋大学博士学位論文 学位の種類 : 博士(医学)(論文) 学位授与年月日:平成9年2月28日 森脇崇之氏の博士論文として提出された","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_12_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第5164号"}]},"item_12_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://dx.doi.org/10.1023/A:1018593805890"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/16683"}]},"item_12_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_12_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1023/A:1018593805890","subitem_relation_type_select":"DOI"}}]},"item_12_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The final publication is available at www.springerlink.com","subitem_rights_language":"en"}]},"item_12_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_12_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0282-0080","subitem_source_identifier_type":"PISSN"}]},"item_12_text_63":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1996"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MORIWAKI, TAKAYUKI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45543","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SUGANUMA, NOBUHIKO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"FURUHASHI, MADOKA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KIKKAWA, FUMITAKA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TOMODA, YUTAKA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45547","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"BOIME, IRVING","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45548","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NAKATA, MUNEHIRO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45549","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MIZUOCHI, TSUGUO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45550","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"o5164.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"o5164.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/14756/files/o5164.pdf"},"version_id":"fe128989-79d5-4a5b-8c32-7bd42b5036f3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"human chorionic gonadotropin","subitem_subject_scheme":"Other"},{"subitem_subject":"hCG β-subunit","subitem_subject_scheme":"Other"},{"subitem_subject":"disulfide bond","subitem_subject_scheme":"Other"},{"subitem_subject":"N-linked oligosaccharide processing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds","subitem_title_language":"en"}]},"item_type_id":"12","owner":"1","path":["1165"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-07-27"},"publish_date":"2012-07-27","publish_status":"0","recid":"14756","relation_version_is_last":true,"title":["Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:37:29.290510+00:00"}