{"created":"2021-03-01T06:21:56.763090+00:00","id":14804,"links":{},"metadata":{"_buckets":{"deposit":"83ff566e-f11b-4981-a51e-852909028000"},"_deposit":{"created_by":17,"id":"14804","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"14804"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00014804","sets":["499:508:509:1403"]},"author_link":["45739","45740","45741","45742","45743","45744","45745","45746","45747","45748","45749","45750","45751"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"271","bibliographicPageStart":"261","bibliographicVolumeNumber":"74","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cytokine-dependent cell lines have been used to analyze the cytokine-induced cellular signaling and the mechanism of oncogenesis. In the current study, we analyzed MOTN-1 and PLT-2 cell lines established from different stages of a T-cell large granular lymphocyte leukemia patient (Daibata et al. 2004). MOTN-1 is IL-2-dependent derived from the chronic phase, whereas IL-2-independent PLT-2 is from the aggressive and terminal stage. They shared considerable chromosome abnormalities and the pattern of T-cell receptor rearrangement, presuming that the cytokine independence of PLT-2 was due to the additive genetic abnormality. Besides IL-2, IL-15 supported MOTN-1 cell growth, because these receptors share b- and g-subunits. IL-2 activated ERK, AKT and STAT pathway of MOTN-1. STAT3 pathway of PLT-2 was also activated by IL-2, suggesting intact IL-2 induces signal transduction of PLT-2. However, ERK1/2 but not AKT, was continuously activated in PLT-2, consistent with the increased Ras-activity of PLT-2. Sequence analysis revealed KRAS G12A mutation but not NRAS and HRAS mutation of PLT-2 but not MOTN-1. Another signaling molecule affecting Ras-signaling pathway, SHP2, which has been frequently mutated in juvenile myelomonocytic leukemia (JMML), did not show mutation. Moreover, MEK inhibitor, PD98059, as well as farnesylation inhibitor inhibited PLT-2 cell growth. Using NIH3T3 and MOTN-1, ERK activation, increased cell proliferation and survival by KRAS G12A were shown, suggesting the important role of KRAS G12A in IL-2-independent growth of PLT-2. Taken together, KRAS G12A is important for IL-2-independent growth of PLT-2 cells and suggests the possibility of involvement of KRAS mutation with disease progression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/16737"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.74.3-4.261","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7434/7434.html","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIZUTANI, NAOKI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ITO, HIROMI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HAGIWARA, KAZUMI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KOBAYASHI, MISA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45742","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HOSHIKAWA, ASUKA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45743","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NISHIDA, YAYOI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45744","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAKAGI, AKIRA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45745","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KOJIMA, TETSUHITO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SUZUKI, MOTOSHI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45747","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OSAWA, YOSUKE","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45748","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OHNISHI, KAZUNORI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45749","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"DAIBATA, MASANORI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45750","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MURATE, TAKASHI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"45751","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"05_Mizutani.pdf","filesize":[{"value":"489.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"05_Mizutani.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/14804/files/05_Mizutani.pdf"},"version_id":"b346ee80-5fa3-4925-beea-e44054908f12"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Human LGL leukemia cell lines","subitem_subject_scheme":"Other"},{"subitem_subject":"MOTN-1 and PLT-2","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-2 independent growth","subitem_subject_scheme":"Other"},{"subitem_subject":"Ras/MAP/ERK pathway","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS G12A mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"Ras activity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"INVOLVEMENT OF KRAS G12A MUTATION IN THE IL-2-INDEPENDENT GROWTH OF A HUMAN T-LGL LEUKEMIA CELL LINE, PLT-2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"INVOLVEMENT OF KRAS G12A MUTATION IN THE IL-2-INDEPENDENT GROWTH OF A HUMAN T-LGL LEUKEMIA CELL LINE, PLT-2","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["1403"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-08-29"},"publish_date":"2012-08-29","publish_status":"0","recid":"14804","relation_version_is_last":true,"title":["INVOLVEMENT OF KRAS G12A MUTATION IN THE IL-2-INDEPENDENT GROWTH OF A HUMAN T-LGL LEUKEMIA CELL LINE, PLT-2"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T06:25:36.246532+00:00"}