@article{oai:nagoya.repo.nii.ac.jp:00015344, author = {SUTO, CHIHARU}, issue = {1-2}, journal = {Nagoya Journal of Medical Science}, month = {Dec}, note = {A virus newly isolated from the smoky-brown cockroach, Periplaneta fuliginosa, was characterized. The virus showed hexagonal structure with a diameter of 19-21 mμ and had a sedimentation value of approximately 110S and a buoyant density of 1.41 g/ml. Neither treatment with chloroform nor heating at 56°C destroyed its infectivity. The virus was stable at pH 3.0. The virus consisted of about 31% of deoxyribonucleic acid and 69% protein. The viral DNA in situ was suggested to be single-stranded by staining property of the virus with acridine orange as well as by reaction to formaldehyde. Furthermore, it was demonstrated that the viral DNA extracted in low salt buffer exhibited single·stranded nature, whereas in high salt buffer double-stranded. Four virion proteins, VP I (M.W., 48,000), VP II (M.W., 52,000), VP III (M.W., 61,000) and VP IV (M.W., 76,000) were detected by SDS-polyacrylamide gel electrophoresis, and they accounted for 6, 58, 30 and 6% of the total viral protein, respectively. These data indicate that the new isolate belongs to the Densovirus of the family Parvoviridae.}, pages = {13--25}, title = {Characterization of a Virus Newly Isolated from the Smoky-Brown Cockroach, Periplaneta Fuliginosa (Serville)}, volume = {42}, year = {1979} }