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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 44(3-4)

Cryopreservation of Human Peripheral Blood Lymphoid Cells Reactive to Allogeneic Antigens, Virus-transformed Autologous Cells and Autologous Leukemia Cells in in vitro Sensitization

https://doi.org/10.18999/nagjms.44.3-4.117
https://doi.org/10.18999/nagjms.44.3-4.117
5d3577b3-c1aa-4d3d-bf75-4f57ef00e420
名前 / ファイル ライセンス アクション
v44n34p117_131.pdf v44n34p117_131.pdf (853.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-01-25
タイトル
タイトル Cryopreservation of Human Peripheral Blood Lymphoid Cells Reactive to Allogeneic Antigens, Virus-transformed Autologous Cells and Autologous Leukemia Cells in in vitro Sensitization
著者 YAMADA, HIRONORI

× YAMADA, HIRONORI

WEKO 46895

YAMADA, HIRONORI

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KODERA, YOSHlHlSA

× KODERA, YOSHlHlSA

WEKO 46896

KODERA, YOSHlHlSA

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YAMADA, KAZUMASA

× YAMADA, KAZUMASA

WEKO 46897

YAMADA, KAZUMASA

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抄録
内容記述 We asked if cryopreservation of human peripheral blood lymphoid cells (PBL) could maintain their function to generate cytotoxic activity against various antigens in vitro. Cells to be frozen were suspended in RPMI 1640 medium supplemented with 10% dimetylsulfoxide and 10% fetal calf serum at a concentration of 1 x 10^7 cells per ml. One and a half ml of this cell solution per polystyrene vial were frozen in an automated programmed freezer at a cooling rate of -1℃ per minute. When used for assays, cells were recovered by rapidly thawing the cell solution in the vials. The ability of thus cryopreserved PBL to generate lymphocyte cytotoxicity was compared to that of fresh PBL from the same individuals. The micromethod of in vitro sensitization was used to generate and measure lymphocyte cytotoxicity in a single 96-well plate. Reactivities to alloantigens were examined in the PBL from 6 individuals and reactivities to Epstein-Barr (E-B) virus-transformed autologous lymphocytes were tested in 4 independent experiments using the PBL from two individuals. In addition, PBL reactivity to autologous leukemia cells was more precisely analysed in one patient with acute myelogenous leukemia. The cytotoxic activities of human PBL directed against alloantigens, E-B virus-transformed autologous lymphocytes, and autologous leukemia cells were satisfactorily recovered after cryopreservation. This encourages the idea to apply this procedure in further analysis of cell-mediated immune reactions in various disease conditions. It may also raise the possibility of producing immune lymphocytes cytotoxic to autologous leukemia cells that could be therapeutically usable for leukemia patients.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.44.3-4.117
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 44, 号 3-4, p. 117-131, 発行日 1982-03
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4434/4434.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17402
識別子タイプ HDL
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