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Some Properties of Amine Oxidase from Soybean Seedlings
https://doi.org/10.18999/nagjms.46.1-4.87
https://doi.org/10.18999/nagjms.46.1-4.87ef19e44a-813e-4ac8-baa7-8fa675e0f230
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-08 | |||||
タイトル | ||||||
タイトル | Some Properties of Amine Oxidase from Soybean Seedlings | |||||
著者 |
MATSUMOTO, TAKATOSHI
× MATSUMOTO, TAKATOSHI× NIMURA, YUJI× FURUTA, TAMAKI× HAYAKAWA, NAOKAZU× ASAI, MASANORI× KUROKAWA, YOSHIE× HATTORI, TATSUO× IYOMASA, YOTARO |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Soybean seedling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amine oxidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diamine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polyamine | |||||
抄録 | ||||||
内容記述 | The purpose of the present study was to clarify the properties of amine oxidase partially purified from soybean seedlings, which the authors used as an enzyme sample for the assay of diamines (putrescine, cadaverine) and polyamines (spermidine, spermine). The enzyme activity was highest with cadaverine followed by putrescine, spermidine, 1,7-diaminoheptane and spermine. The optimum pH was 7.8 for putrescine and cadaverine and 8.0 for spermidine and spermine. Carbonyl reagents, such as semicarbazide, and chelating reagents, such as cuprizone, were effective inhibitots of soybean seedling amine oxidase (SSAO), suggesting that Cu++ and pyridoxal may be cofactors for SSAO. Therefore, SSAO appeared to be a diamine oxidase (EC 1.4.3.6). It was stable at 50°C for at least 2 hours, and the apparent Km values for all diamines and polyamines were extremely low. Since SSAO is present in great quantities in soybean seedlings, which can be easily obtained in every season in Japan, it is very suitable as an enzyme sample for the assay of diamines and polyamines. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.46.1-4.87 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4614/4614.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
Nagoya Journal of Medical Science 巻 46, 号 1-4, p. 87-94, 発行日 1984-03 |
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著者版フラグ | ||||||
値 | publisher |