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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 46(1-4)

Some Properties of Amine Oxidase from Soybean Seedlings

https://doi.org/10.18999/nagjms.46.1-4.87
https://doi.org/10.18999/nagjms.46.1-4.87
ef19e44a-813e-4ac8-baa7-8fa675e0f230
名前 / ファイル ライセンス アクション
v46n14p87_94.pdf v46n14p87_94.pdf (474.0 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-02-08
タイトル
タイトル Some Properties of Amine Oxidase from Soybean Seedlings
著者 MATSUMOTO, TAKATOSHI

× MATSUMOTO, TAKATOSHI

WEKO 98713

MATSUMOTO, TAKATOSHI

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NIMURA, YUJI

× NIMURA, YUJI

WEKO 98714

NIMURA, YUJI

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FURUTA, TAMAKI

× FURUTA, TAMAKI

WEKO 98715

FURUTA, TAMAKI

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HAYAKAWA, NAOKAZU

× HAYAKAWA, NAOKAZU

WEKO 98716

HAYAKAWA, NAOKAZU

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ASAI, MASANORI

× ASAI, MASANORI

WEKO 98717

ASAI, MASANORI

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KUROKAWA, YOSHIE

× KUROKAWA, YOSHIE

WEKO 98718

KUROKAWA, YOSHIE

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HATTORI, TATSUO

× HATTORI, TATSUO

WEKO 98719

HATTORI, TATSUO

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IYOMASA, YOTARO

× IYOMASA, YOTARO

WEKO 98720

IYOMASA, YOTARO

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キーワード
主題Scheme Other
主題 Soybean seedling
キーワード
主題Scheme Other
主題 Amine oxidase
キーワード
主題Scheme Other
主題 Diamine
キーワード
主題Scheme Other
主題 Polyamine
抄録
内容記述 The purpose of the present study was to clarify the properties of amine oxidase partially purified from soybean seedlings, which the authors used as an enzyme sample for the assay of diamines (putrescine, cadaverine) and polyamines (spermidine, spermine). The enzyme activity was highest with cadaverine followed by putrescine, spermidine, 1,7-diaminoheptane and spermine. The optimum pH was 7.8 for putrescine and cadaverine and 8.0 for spermidine and spermine. Carbonyl reagents, such as semicarbazide, and chelating reagents, such as cuprizone, were effective inhibitots of soybean seedling amine oxidase (SSAO), suggesting that Cu++ and pyridoxal may be cofactors for SSAO. Therefore, SSAO appeared to be a diamine oxidase (EC 1.4.3.6). It was stable at 50°C for at least 2 hours, and the apparent Km values for all diamines and polyamines were extremely low. Since SSAO is present in great quantities in soybean seedlings, which can be easily obtained in every season in Japan, it is very suitable as an enzyme sample for the assay of diamines and polyamines.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.46.1-4.87
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4614/4614.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 46, 号 1-4, p. 87-94, 発行日 1984-03
著者版フラグ
値 publisher
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