{"created":"2021-03-01T06:22:40.898611+00:00","id":15490,"links":{},"metadata":{"_buckets":{"deposit":"b3390bcc-9381-4015-97db-36df99c93ff2"},"_deposit":{"created_by":17,"id":"15490","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"15490"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00015490","sets":["499:508:509:1462"]},"author_link":["47230","47231","47232","47233","47234"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"34","bibliographicPageStart":"25","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The mode of natural killer (NK) cell migration to the sites of inflammation and transplanted tumors was investigated by using dry ice for physical irritation and 1-fluoro-2,4 dinitrobenzene (DNFB) for chemical irritation in mouse ear. In experiments with transplanted tumors, NK cell sensitive tumor cells (RL♂I) and insensitive tumor cells (p815) were transplanted into the ears of C3H and BALB/c mice, respectively. Employing a polyclonal rabbit antiserum against asialoGM1 (GA1), and a monoclonal rat antiserum against Thy-1 in an immunohistochemical double-staining technique, we enumerated the number of Thy-1-positive and asialoGM1- positive (Thy-1^+GA1^+) cells and Thy-1-negative and asialoGM1-positive (Thy-1^-GA1^+) cells at various times of irritation. Following physical irritation, Thy-1^-GA1^+ cells (108.8 ± 4.5/mm2 at 24 hand 71.2 ± 3.8/mm2 at 48 h) were found in the epidermis, whereas Thy-1^+GA1^+ cells were not found. In delayed-type skin reaciton by DNFB, Thy-1^-GA1^+ cells (87.1 ± 5.8/mm2 at 24h and 60.7 ± 2.9/mm2 at 48h) and Thy-1^+GA1^+ cells (26.4 ± 3.6/mm2 at 24h and 15.3 ± 4.3/mm2 at 48h) were found in the dermis. Since it was reported by previous investigators that Thy-1^+GA1^+ cells are NK cells, we assumed that NK cells infiltrated nonspecifically in the dermis in delayed-type skin reaction by DNFB. In the tumor transplant experiments. the GA1^+ cells were found near both types of tumors, but they were in contact with RL♂1 and not with p815. Because it was reported that GA1^+ monocytes do not have cytotoxicity against tumor cells, our findings suggest that GA1^+ cells migrate nonspecifically to the sites of inflammation, and that the NK cells among them may make direct contact with the tumor cells when they encounter NK cell sensitive tumors.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/17508"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.51.1-4.25","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5114/5114.html","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAHAR, LUTFUN","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47230","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KONDO, TAKAO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47231","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HABU, SONOKO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47232","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OHASHI, MASARU","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47233","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NAKANE, PAUL K.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47234","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"v51n14p25_34.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v51n14p25_34.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/15490/files/v51n14p25_34.pdf"},"version_id":"9fc93a92-11bf-4a56-a05a-4b55615dff7e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"NK CELLS","subitem_subject_scheme":"Other"},{"subitem_subject":"MOUSE SKIN","subitem_subject_scheme":"Other"},{"subitem_subject":"IMMUNOHISTOCHEMISTRY","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Natural Killer Cells in Inflammatory Lesions and Transplanted Tumors in Mouse Skin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Natural Killer Cells in Inflammatory Lesions and Transplanted Tumors in Mouse Skin","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["1462"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-13"},"publish_date":"2013-02-13","publish_status":"0","recid":"15490","relation_version_is_last":true,"title":["Natural Killer Cells in Inflammatory Lesions and Transplanted Tumors in Mouse Skin"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T05:50:39.978186+00:00"}