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Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate
https://doi.org/10.18999/nagjms.52.1-4.5
https://doi.org/10.18999/nagjms.52.1-4.5f75dc6d9-1f7c-4565-b274-b48058843a72
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-14 | |||||
タイトル | ||||||
タイトル | Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate | |||||
言語 | en | |||||
著者 |
CHEN, FAN
× CHEN, FAN× GOTO, SETSUKO× NAWA, AKIHIRO× OKAMOTO, TOMOMITSU× TOMODA, YUTAKA |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Epidermal Growth Factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Receptor Binding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Choriocarcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Actinomycin-D | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Methotrexate | |||||
抄録 | ||||||
内容記述 | Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37°C resulted in a higher binding than that at 22°C or at 4°C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.52.1-4.5 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5214/5214.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 52, 号 1-4, p. 5-11, 発行日 1990-03 |
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著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/17510 | |||||
識別子タイプ | HDL |