{"_buckets": {"deposit": "91ae9ace-3c00-4047-abf2-f731f9a2d712"}, "_deposit": {"id": "15492", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "15492"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00015492"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1990-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-4", "bibliographicPageEnd": "11", "bibliographicPageStart": "5", "bibliographicVolumeNumber": "52", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37\u00b0C resulted in a higher binding than that at 22\u00b0C or at 4\u00b0C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5214/5214.html"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/17510"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.52.1-4.5", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN\uff08Online\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "ISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "CHEN, FAN"}], "nameIdentifiers": [{"nameIdentifier": "47237", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "GOTO, SETSUKO"}], "nameIdentifiers": [{"nameIdentifier": "47238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAWA, AKIHIRO"}], "nameIdentifiers": [{"nameIdentifier": "47239", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OKAMOTO, TOMOMITSU"}], "nameIdentifiers": [{"nameIdentifier": "47240", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TOMODA, YUTAKA"}], "nameIdentifiers": [{"nameIdentifier": "47241", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v52n14p5_11.pdf", "filesize": [{"value": "452.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 452400.0, "url": {"label": "v52n14p5_11.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/15492/files/v52n14p5_11.pdf"}, "version_id": "b4eb2545-c7cf-4e68-905e-0eb09f04cb3a"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Epidermal Growth Factor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Receptor Binding", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Choriocarcinoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Actinomycin-D", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Methotrexate", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/1463"], "permalink_uri": "https://doi.org/10.18999/nagjms.52.1-4.5", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2013-02-14"}, "publish_date": "2013-02-14", "publish_status": "0", "recid": "15492", "relation": {}, "relation_version_is_last": true, "title": ["Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate"], "weko_shared_id": 3}