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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 52(1-4)

Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate

https://doi.org/10.18999/nagjms.52.1-4.5
https://doi.org/10.18999/nagjms.52.1-4.5
f75dc6d9-1f7c-4565-b274-b48058843a72
名前 / ファイル ライセンス アクション
v52n14p5_11.pdf v52n14p5_11.pdf (452.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-02-14
タイトル
タイトル Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate
著者 CHEN, FAN

× CHEN, FAN

WEKO 47237

CHEN, FAN

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GOTO, SETSUKO

× GOTO, SETSUKO

WEKO 47238

GOTO, SETSUKO

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NAWA, AKIHIRO

× NAWA, AKIHIRO

WEKO 47239

NAWA, AKIHIRO

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OKAMOTO, TOMOMITSU

× OKAMOTO, TOMOMITSU

WEKO 47240

OKAMOTO, TOMOMITSU

Search repository
TOMODA, YUTAKA

× TOMODA, YUTAKA

WEKO 47241

TOMODA, YUTAKA

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キーワード
主題Scheme Other
主題 Epidermal Growth Factor
キーワード
主題Scheme Other
主題 Receptor Binding
キーワード
主題Scheme Other
主題 Choriocarcinoma
キーワード
主題Scheme Other
主題 Actinomycin-D
キーワード
主題Scheme Other
主題 Methotrexate
抄録
内容記述 Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37°C resulted in a higher binding than that at 22°C or at 4°C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.52.1-4.5
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 52, 号 1-4, p. 5-11, 発行日 1990-03
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5214/5214.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17510
識別子タイプ HDL
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