{"_buckets": {"deposit": "91ae9ace-3c00-4047-abf2-f731f9a2d712"}, "_deposit": {"created_by": 17, "id": "15492", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "15492"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00015492", "sets": []}, "author_link": ["47237", "47238", "47239", "47240", "47241"], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1990-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-4", "bibliographicPageEnd": "11", "bibliographicPageStart": "5", "bibliographicVolumeNumber": "52", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37°C resulted in a higher binding than that at 22°C or at 4°C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/17510"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.52.1-4.5", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", 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"nameIdentifiers": [{"nameIdentifier": "47237", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "GOTO, SETSUKO", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "47238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAWA, AKIHIRO", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "47239", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OKAMOTO, TOMOMITSU", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "47240", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TOMODA, YUTAKA", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "47241", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, 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{"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "17", "path": ["1463"], "permalink_uri": "https://doi.org/10.18999/nagjms.52.1-4.5", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-02-14"}, "publish_date": "2013-02-14", "publish_status": "0", "recid": "15492", "relation": {}, "relation_version_is_last": true, "title": ["Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate"], "weko_shared_id": -1}