{"created":"2021-03-01T06:22:41.025318+00:00","id":15492,"links":{},"metadata":{"_buckets":{"deposit":"91ae9ace-3c00-4047-abf2-f731f9a2d712"},"_deposit":{"created_by":17,"id":"15492","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"15492"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00015492","sets":["499:508:509:1463"]},"author_link":["47237","47238","47239","47240","47241"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"11","bibliographicPageStart":"5","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37°C resulted in a higher binding than that at 22°C or at 4°C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/17510"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.52.1-4.5","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5214/5214.html","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"CHEN, FAN","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47237","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"GOTO, SETSUKO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47238","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NAWA, AKIHIRO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OKAMOTO, TOMOMITSU","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TOMODA, YUTAKA","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47241","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"v52n14p5_11.pdf","filesize":[{"value":"452.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v52n14p5_11.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/15492/files/v52n14p5_11.pdf"},"version_id":"b4eb2545-c7cf-4e68-905e-0eb09f04cb3a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Epidermal Growth Factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Receptor Binding","subitem_subject_scheme":"Other"},{"subitem_subject":"Choriocarcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Actinomycin-D","subitem_subject_scheme":"Other"},{"subitem_subject":"Methotrexate","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["1463"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-14"},"publish_date":"2013-02-14","publish_status":"0","recid":"15492","relation_version_is_last":true,"title":["Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T05:50:45.701580+00:00"}