{"created":"2021-03-01T06:22:47.144703+00:00","id":15587,"links":{},"metadata":{"_buckets":{"deposit":"c4052d46-b8b0-4f19-8d54-9f11b3b7e7d6"},"_deposit":{"created_by":17,"id":"15587","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"15587"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00015587","sets":["499:508:509:1468"]},"author_link":["47495","47496","47497","47498","47499","47500","47501"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"111","bibliographicPageStart":"101","bibliographicVolumeNumber":"75","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Autologous chondrocyte implantation (ACI) is a method of cartilage repair. To improve the quality of regenerated tissue by ACI, it is essential to identify surface marker expression correlated with the differentiation status of monolayer expanded human articular chondrocytes and to define the index for discriminating dedifferentiated cells from monolayer expanded human articular chondrocytes. Normal human articular chondrocytes were cultured in monolayer until passage 4. At each passage, mRNA expression of collagen type I, II, and X and aggrecan was analyzed by real-time quantitative PCR, and the surface marker expression of CD14, CD26, CD44, CD49a, CD49c, CD54, and CD151 was analyzed by fluorescenceactivated cell sorting (FACS). The ratios of mRNA levels of collagen type II to I (Col II/Col I) represented the differentiation status of chondrocytes more appropriately during monolayer culture. The surface marker expression of CD44, CD49c, and CD151 was upregulated according to the dedifferentiation status, whereas that of CD14, CD49a, and CD54 was downregulated. The most appropriate combination of the ratio of Col II/Col I was CD54 and CD44. Cell sorting was performed using a magnetic cell sorting system (MACS) according to CD54 and CD44, and real-time quantitative PCR was performed for the cell subpopulations before and after cell sorting. The expression of collagen type II and aggrecan of the chondrocytes after MACS was higher than that before sorting, but not significantly. The mean fluorescence intensity (MFI) ratio of CD54 to CD44 could be an adequate candidate as the index of the differentiation status.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/17606"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.75.1-2.101","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7512/7512.html","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HAMADA, TAKASHI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47495","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SAKAI, TADAHIRO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47496","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HIRAIWA, HIDEKI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47497","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"NAKASHIMA, MOTOSHIGE","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47498","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ONO, YOHEI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47499","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MITSUYAMA, HIROHITO","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47500","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISHIGURO, NAOKI","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"47501","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"11_Hamada.pdf","filesize":[{"value":"843.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"11_Hamada.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/15587/files/11_Hamada.pdf"},"version_id":"0d672873-121f-4378-b0bd-4880923a107d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Autologous chondrocyte implantation","subitem_subject_scheme":"Other"},{"subitem_subject":"surface marker","subitem_subject_scheme":"Other"},{"subitem_subject":"differentiation index","subitem_subject_scheme":"Other"},{"subitem_subject":"CD44","subitem_subject_scheme":"Other"},{"subitem_subject":"ICAM-1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["1468"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-03-01"},"publish_date":"2013-03-01","publish_status":"0","recid":"15587","relation_version_is_last":true,"title":["Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T05:38:04.383668+00:00"}