{"created":"2021-03-01T06:23:22.642093+00:00","id":16140,"links":{},"metadata":{"_buckets":{"deposit":"88162a25-d6d6-4492-ac8c-2b94ea82f0aa"},"_deposit":{"id":"16140","owners":[],"pid":{"revision_id":0,"type":"depid","value":"16140"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00016140","sets":["879:1183:1184:1497"]},"author_link":["83540","83541"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Bone sample preparation method for 14C dating","subitem_alternative_title_language":"en"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"189","bibliographicPageStart":"185","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"名古屋大学加速器質量分析計業績報告書","bibliographic_titleLang":"ja"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bones are one of the most attractive materials for archaeological dating, because they often provide direct information about prehistoric events. Bone collagen is commonly used for 14C-dating of bones. The bone pretreatment includes the removal of mineral phase of bones with 0.4-0.6M HCl followed by a gentle-base-acid extraction to remove extraneous carbon such as humic acids from the organic bone residue. Next, the collagen is dissolved as gelatin at 85-90℃ and pH 3 and, and filtered through a glass filter to remove non-soluble contaminants, then freeze-dried. The gelatin with its yield of <0.7 wt% could remain exogenous carbon, and so more cleaning step using the high molecular weight (>30 kD) of gelatins to remove contaminants by ultrafiltration is needed. The appropriate schematic overview of collagen extraction and ultrafiltration is performed. ","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"遺跡から出土する骨は､当時の人の食性や生活環境､年代などの情報を直接与えてくれるものとして､考古学において非常に重要である。骨の14C年代測定においては､一般的に､硬タンパク質の骨コラーゲンが用いられる。骨は､まず0.4-0.6M HClによって､無機成分を除去する｡得られた酸不溶脱灰成分に0.1MNaOH､0.1MHClにより穏やかにアルカリ･酸処理を行ない､フミン酸などの外来炭素を除去する。次に､85-90℃､pH3の弱酸性の水にコラーゲンを溶解し､ゼラチン抽出を行なう｡ゼラチン溶液はガラスフィルターによるろ過を行ない､不溶性の外来炭素を除去する｡得られたゼラチン収率が0.7wt%よりも低い場合は､外来炭素が完全に除去されていない可能性が考えられ､さらに限外ろ過によって､3万分子量以上の高分子ゼラチンのみをふるい分けする必要がある｡本稿においては､以上の骨コラーゲン抽出法､およびゼラチンの限外ろ過法に関して､現在最も適切と考えられる手順について紹介する｡","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/sumrua.23.185","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋大学年代測定資料研究センター","subitem_publisher_language":"ja"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"南, 雅代","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"83540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minami, Masayo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"83541","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"minami2011.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"minami2011.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/16140/files/minami2011.pdf"},"version_id":"12f788c9-a10c-4d0a-be56-631f8b1bf05d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨","subitem_subject_scheme":"Other"},{"subitem_subject":"放射性炭素年代","subitem_subject_scheme":"Other"},{"subitem_subject":"試料調整法","subitem_subject_scheme":"Other"},{"subitem_subject":"bone","subitem_subject_scheme":"Other"},{"subitem_subject":"14C age","subitem_subject_scheme":"Other"},{"subitem_subject":"sample preparation method","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"14C年代測定のための骨試料調整法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"14C年代測定のための骨試料調整法","subitem_title_language":"ja"}]},"item_type_id":"9","owner":"1","path":["1497"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-06-07"},"publish_date":"2013-06-07","publish_status":"0","recid":"16140","relation_version_is_last":true,"title":["14C年代測定のための骨試料調整法"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:03:49.834878+00:00"}