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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 75(3-4)

SIMPLE AND RAPID ASSAY METHOD FOR SIMULTANEOUS QUANTIFICATION OF URINARY NICOTINE AND COTININE USING MICRO-EXTRACTION BY PACKED SORBENT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY

https://doi.org/10.18999/nagjms.75.3-4.255
https://doi.org/10.18999/nagjms.75.3-4.255
fe8891d2-4b3e-4e6e-9f44-126dd23834c2
名前 / ファイル ライセンス アクション
11_Ogawa.pdf 11_Ogawa.pdf (193.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-09-12
タイトル
タイトル SIMPLE AND RAPID ASSAY METHOD FOR SIMULTANEOUS QUANTIFICATION OF URINARY NICOTINE AND COTININE USING MICRO-EXTRACTION BY PACKED SORBENT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY
著者 Iwai, Masae

× Iwai, Masae

WEKO 49253

Iwai, Masae

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Ogawa, Tadashi

× Ogawa, Tadashi

WEKO 49254

Ogawa, Tadashi

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Hattori, Hideki

× Hattori, Hideki

WEKO 49255

Hattori, Hideki

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Zaitsu, Kei

× Zaitsu, Kei

WEKO 49256

Zaitsu, Kei

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Ishii, Akira

× Ishii, Akira

WEKO 49257

Ishii, Akira

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Suzuki, Osamu

× Suzuki, Osamu

WEKO 49258

Suzuki, Osamu

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Seno, Hiroshi

× Seno, Hiroshi

WEKO 49259

Seno, Hiroshi

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キーワード
主題Scheme Other
主題 micro-extraction by packed sorbent (MEPS)
キーワード
主題Scheme Other
主題 gas chromatography-mass spectrometry (GC-MS)
キーワード
主題Scheme Other
主題 nicotine
キーワード
主題Scheme Other
主題 cotinine
抄録
内容記述 A simple and rapid method for determination of nicotine and cotinine levels in urine was developed using samples prepared by micro-extraction by packed sorbent (MEPS) and subjected to gas chromatography- mass spectrometry (GC-MS) analysis. This method provided good reproducibility, as well as good linearity of calibration curves in the range of 1–100 and 50–1000 ng/mL for quality control samples spiked with nicotine and cotinine, respectively. The detection limit of nicotine and cotinine was as low as 0.25 and 20 ng/mL, respectively. An evaporation procedure is not suitable for nicotine determination, thus an advantage of the present MEPS assay method is direct testing with GC-MS without the need for evaporation to a dry solvent. Our findings show that it may be useful for determining nicotine levels in various types of research studies.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.75.3-4.255
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 75, 号 3-4, p. 255-261, 発行日 2013-08
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7534/7534.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/18475
識別子タイプ HDL
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