@article{oai:nagoya.repo.nii.ac.jp:00016505, author = {SHIOYA, MASAO}, issue = {2}, journal = {Nagoya Journal of Medical Science}, month = {Sep}, note = {In this paper, an attempt to devise a most satisfactory method of thromboplastin generation test (TGT) was made and the results were as follows : 1) As anticoagulant for the preparation of plasma, sodium citrate is superior to potassium oxalate, because the various factors in the former are more stable than those in oxalated plasma. 2) In adsorption of plasma, BaSO4 and Al(OH)3-gel are both useful. In citrated plasma, the optimum dose was 200 mg/ml and preferable adsorption time at 37°C ranged from 5 to 20 minutes with BaSO4,, and with AL(OH)3 0.03 ml/ml, 1-3 minutes. However, there is need to use BaSO4, of purity above 99% and with grain size mostly of 1-2µ 3) Serum should be separated 1 hour after initiation of the incubation in case of normal whole blood clotting time, and 3 hours after in hemophiliacs. There is no need to activate the serum with glass beads. 4) The optimum concentration of platelet suspension was found to be 200,000- 300,000/cmm and washing of platelets should be made for at least 4 times. But in general, platelet substitutes should be used, and the potency of such substitutes should preferably possess minimal substrate clotting time of about 10 seconds. 5) The normal value of TGT was 9.97± 0.938 seconds for the minimal substrate clotting time, or 100± 23.3% in thromboplastic activity. 6) The standard dilution curve of TGT can be plotted linearly on a bilogarithmic scale and they should be prepared for each time of measurement, and the results of TGT indicated by percentages of the normal value.}, pages = {193--209}, title = {Studies on Thromboplastin Generation Test}, volume = {30}, year = {1967} }