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Insulin treatment on alloxan diabetic rats improved both indexes up to the normal levels. However, insulin addition in vitro into mitochondria suspension had no effect on the both indexes. It was found that insulin addition in vitro blocks the uncoupled respiration induced by 2, 4-dinitrophenol. One of insulin actions, a disulfide hormone, in vivo was proposed to protect mitochondria from uncoupling factor in connection with thioldisulfide interchange to produce the conformational in mitochodria membrane. 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