@article{oai:nagoya.repo.nii.ac.jp:00016595,
 author = {KATSUMATA, KAZUO and YAMANAKA, NAOKI and OZAWA, TAKAYUKI and KATSUMATA, YOSHINAO},
 issue = {2},
 journal = {Nagoya Journal of Medical Science},
 month = {Jan},
 note = {Male albino (Wistar strain) rats were fed with ordinary laboratory chew, Oriental pellet, properly balanced with regard to protein, lipids, carbohydrates, minerals, and vitamins, and with free water supply for ten days. Thereafter, the rats were force-fed daily with 4.5 ml of 30% ethanol solution or glucose solution, isocaloric with the former, by a gastric tube for another two weeks. The respiratory control index and AMP/ 0 ratio, the parameters which represent the intactness of mitochondria and the efficiency of the oxidative phosphorylation of liver mitochondria, were measured with liver mitochondria of the control and the alcohol-fed rats. Both indexes were found to be significantly lowered in the alcohol-fed rats indicating that ethanol feeding causes partial uncoupling of the oxidative phosphorylation in the liver mitochondria. The addition of bovine serum albumin in vitro did not recover this partial uncoupling. In the alcohol-fed rats for two weeks, the concentration of NEFA, ethanol, and acetaldehyde in the serum were increased which suggests that the increase has close relation with the disturbance of mitochondrial functions in alcohol fed rats, as NEFA is one of the well-known uncouplers. In order to elucidate the effects of alcohol and acetaldehyde on the mitochondrial oxidative phosphorylation in vitro, different amounts of alcohol or acetaldehyde were added to the mitochondrial suspension of the control group. Addition of more than 40 μmoles of ethanol/ml of medium or more than 10 μmoles of acetaldehyde/ml of medium impaired both the respiratory control index and AMP/O ratio. It is noted that the minimum concentration of ethanol effective for the isolated mitochondrial respiratory control and AMP/ 0 ratio was 40 μmoles/ml, the concentration which could be observed in the serum of the alcohol fed rats in vivo, but that of acetaldehyde was too high to be observed in ethanol fed rats in vivo.},
 pages = {347--357},
 title = {The Mitochondrial Respiratory Control and Oxidative Phosphorylation of Ethanol Fed Rats},
 volume = {32},
 year = {1970}
}