@article{oai:nagoya.repo.nii.ac.jp:00016632, author = {NIIMI, MORIHIKO}, issue = {4}, journal = {Nagoya Journal of Medical Science}, month = {Mar}, note = {The present electron microscopic study was attempted to demonstrate a detailed localization of acid and alkaline phosphatase within the phagocytosing rabbit heterophils and further to clarify the difference in time course between the appearance of acid phosphatase and of alkaline phosphatase reaction products in the phagocytic vacuoles. As material, rabbit bone marrow cell suspension and zymosan for the phagocytized particle were used. Alkaline phosphatase reaction was seen in relatively small granules (i.e. specific granules), and acid phosphatase reaction in large granules (i.e. azurophilic granules). Alkaline phosphatase fused into the phagocytic vacuoles earlier than acid phosphatase. These distinctly different patterns of enzyme fusion might be dependent on the qualitative heterogeneity of granules and also on the quantitative difference between granules with acid phosphatase and those with alkaline phosphatase of mature heterophils which show most active phagocytosis among cells with varying maturation.}, pages = {287--294}, title = {A Cytochemical Electron Microscopic Study of Rabbit Heterophilic Leukocytes during Phagocytosis Acid and Alkaline Phosphatase Reactions}, volume = {33}, year = {1971} }