@article{oai:nagoya.repo.nii.ac.jp:00016652,
 author = {TAKAHASHI, YOHEI},
 issue = {3},
 journal = {Nagoya Journal of Medical Science},
 month = {Feb},
 note = {Configurational changes of the mitochondrial inner ·membrane system were examined ultrastructurally and biochemically. Mitochondria in intact heart muscle appeared predominantly in a so-called "orthodox" mode. On the other hand, the mitochondria isolated from liver or heart muscle showed a so-called "aggregated" mode. Many authors who have examined extensively isolated mitochondria found that they underwent ultrastructural changes coupled with their functional metabolic states, corresdonding to the surrounding conditions such as substrates, Pi, nucleotides or uncouplers. Intact heart muscles were fixed by perfusion with glutaraldehyde solutions, and two types of configurational states of mitochondria were found. The first type of mitochondria showed an irregular and relatively straight arrangement of the inner membrane system. The second had an angular and regular configuration. Ehrlich ascites tumor cells also exhibited the twisting and waving con- figuration of mitochondrial inner membrane when fixed at the state of respiratory inhibition induced by addition of glucose. Mitochondria of fasted ascites cells showed an irregular configuration. Mitochondria in the rough homogenates of rat heart muscle had morphologically a transitional structure between isolated mitochondria and in situ ones. Mitochondria in the homogenates fixed at various functional metabolic states showed particular configurational changes correlating to their functional states in both "orthodox" and "aggregated" modas. From in vitro experiments, the angular or twisted configuration of the inner membrane of mitochondria was found to be induced by oxidizable substrates and Pi. As intact cells possess Pi endogenously, the mitochondria in situ are considered to have two different configurations.},
 pages = {213--226},
 title = {Studies on the Structure and Function of Biological Membrane System : II. Configurational Changes of Mitochondria in situ Correlating to Functional States},
 volume = {34},
 year = {1972}
}