{"_buckets": {"deposit": "561cc44e-bbe2-44b9-a7d8-b843338908b5"}, "_deposit": {"id": "16789", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "16789"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00016789", "sets": ["1165"]}, "author_link": ["49831", "49832", "49833", "49834", "49835", "49836", "49837"], "item_12_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageEnd": "e45056", "bibliographicPageStart": "e45056", "bibliographicVolumeNumber": "7", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE", "bibliographic_titleLang": "en"}]}]}, "item_12_date_granted_64": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2012-09-11"}]}, "item_12_degree_grantor_62": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "名古屋大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "Nagoya University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13901", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_12_degree_name_61": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)", "subitem_degreename_language": "ja"}]}, "item_12_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "It is suggested that migration of airway smooth muscle (ASM) cells plays an important role in the pathogenesis of airway remodeling in asthma. Increases in intracellular Ca2+ concentrations ([Ca2+]i) regulate most ASM cell functions related to asthma, such as contraction and proliferation. Recently, STIM1 was identified as a sarcoplasmic reticulum (SR) Ca2+ sensor that activates Orai1, the Ca2+ channel responsible for store-operated Ca2+ entry (SOCE). We investigated the role of STIM1 in [Ca2+]i and cell migration induced by platelet-derived growth factor (PDGF)-BB in human ASM cells. Cell migration was assessed by a chemotaxis chamber assay. Human ASM cells express STIM1, STIM2, and Orai1 mRNAs. SOCE activated by thapsigargin, an inhibitor of SR Ca2+-ATPase, was significantly blocked by STIM1 siRNA and Orai1 siRNA but not by STIM2 siRNA. PDGF-BB induced a transient increase in [Ca2+]i followed by sustained [Ca2+]i elevation. Sustained increases in [Ca2+]i due to PDGF-BB were significantly inhibited by a Ca2+ chelating agent EGTA or by siRNA for STIM1 or Orai1. The numbers of migrating cells were significantly increased by PDGF-BB treatment for 6 h. Knockdown of STIM1 and Orai1 by siRNA transfection inhibited PDGF-induced cell migration. Similarly, EGTA significantly inhibited PDGF-induced cell migration. In contrast, transfection with siRNA for STIM2 did not inhibit the sustained elevation of [Ca2+]i or cell migration induced by PDGF-BB. These results demonstrate that STIM1 and Orai1 are essential for PDGF-induced cell migration and Ca2+ influx in human ASM cells. STIM1 could be an important molecule responsible for airway remodeling.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_12_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成25年3月25日 菅沼伸一氏の博士論文として提出された", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_12_dissertation_number_65": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第9966号"}]}, "item_12_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://dx.doi.org/10.1371/journal.pone.0045056"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/18819"}]}, "item_12_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "PLOS ONE", "subitem_publisher_language": "en"}]}, "item_12_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1371/journal.pone.0045056", "subitem_relation_type_select": "DOI"}}]}, "item_12_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "(C) 2012 Suganuma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.", "subitem_rights_language": "en"}]}, "item_12_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_12_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "EISSN"}]}, "item_12_text_63": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2012"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Suganuma, Nobukazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49831", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Satoru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49832", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aso, Hiromichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49833", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kondo, Masashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49834", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Mitsuo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49835", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sokabe, Masahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49836", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa, Yoshinori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49837", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "k9966.pdf", "filesize": [{"value": "544.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 544800.0, "url": {"label": "k9966.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/16789/files/k9966.pdf"}, "version_id": "39bd3de0-08c6-4dad-9cd1-0aa4d6b24bbc"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "STIM1 Regulates Platelet-Derived Growth Factor-Induced Migration and Ca2+ Influx in Human Airway Smooth Muscle Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "STIM1 Regulates Platelet-Derived Growth Factor-Induced Migration and Ca2+ Influx in Human Airway Smooth Muscle Cells", "subitem_title_language": "en"}]}, "item_type_id": "12", "owner": "1", "path": ["1165"], "permalink_uri": "http://hdl.handle.net/2237/18819", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-11-06"}, "publish_date": "2013-11-06", "publish_status": "0", "recid": "16789", "relation": {}, "relation_version_is_last": true, "title": ["STIM1 Regulates Platelet-Derived Growth Factor-Induced Migration and Ca2+ Influx in Human Airway Smooth Muscle Cells"], "weko_shared_id": -1}