WEKO3
アイテム
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Mesenchymal stromal cells of human umbilical cord Wharton's jelly accelerate wound healing by paracrine mechanisms
http://hdl.handle.net/2237/18891
http://hdl.handle.net/2237/188917968e696-05a2-40c9-bcb4-397b319be56b
名前 / ファイル | ライセンス | アクション |
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k9936_thesis.pdf (1.4 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2013-11-15 | |||||
タイトル | ||||||
タイトル | Mesenchymal stromal cells of human umbilical cord Wharton's jelly accelerate wound healing by paracrine mechanisms | |||||
言語 | en | |||||
著者 |
Shohara, Ryutaro
× Shohara, Ryutaro× Yamamoto, Akihito× Takikawa, Sachiko× Iwase, Akira× Hibi, Hideharu× Kikkawa, Fumitaka× Ueda, Minoru |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is the author's version of a work that was accepted for publication in Cytotherapy. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cytotherapy. v.14, n.10, 2012, p.1171-1181 DOI: 10.3109/14653249.2012.706705 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human umbilical cord perivascular cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | wound healing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mesenchymal stromal cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | anti-inflammatory M2 macrophage | |||||
抄録 | ||||||
内容記述 | Background aims. Mesenchymal stromal cells (MSC) can be isolated from the perivascular connective tissue of umbilical cords, called Wharton's jelly. These human umbilical cord perivascular cells (HUCPVC) might provide therapeutic benefits when treating skeletal or cutaneous malformations in neonatal patients. Methods. HUCPVC were isolated, and their proliferation rate, marker expression and multilineage differentiation potential determined. HUCPVC or their conditioned medium (HUCPVC-CM) was injected into the excisional wound of a mouse splinted-wound model. The effects of the treatment on wound closure were examined by morphohistochemical and gene expression analyses. Results. HUCPVC expressed typical MSC markers and could differentiate into osteoblastic and adipogenic lineages. HUCPVC transplanted into the mouse wound accelerated wound closure. Immunohistologic analysis showed that the HUCPVC accelerated wound healing by enhancing collagen deposition and angiogenesis via paracrine mechanisms. Furthermore, treatment with HUCPVC-CM alone significantly enhanced wound closure. HUCPVC-CM increased the number of anti-inflammatory M2 macrophages expressing resistin-like molecule (RELM)-α/CD11b and promoted neovessel maturation. Quantitative polymerase chain reaction (PCR) analysis showed that HUCPVC-CM increased the expression of tissue-repairing cytokines interleukin (IL)-10, transforming growth factor (TGF)-β1, vascular endothelial growth factor (VEGF)-1 and angiopoietin-1 at the healing wound. Conclusions. Our results show that HUCPVC promotes wound healing via multifaceted paracrine mechanisms. Together with their ability to differentiate into the osteogenic linage, HUCPVC may provide significant therapeutic benefits for treating wounds in neonatal patients. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成25年1月31日 匠原龍太郎氏の博士論文として提出された | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Informa healthcare | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_db06 | |||||
タイプ | doctoral thesis | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3109/14653249.2012.706705 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1465-3249 | |||||
書誌情報 |
en : Cytotherapy 巻 14, 号 10, p. 1171-1181, 発行日 2012-09 |
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学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 13901 | |||||
言語 | ja | |||||
学位授与機関名 | 名古屋大学 | |||||
言語 | en | |||||
学位授与機関名 | Nagoya University | |||||
学位授与年度 | ||||||
学位授与年度 | 2012 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2012-09-01 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第9936号 | |||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://dx.doi.org/10.3109/14653249.2012.706705 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/18891 | |||||
識別子タイプ | HDL |