@article{oai:nagoya.repo.nii.ac.jp:00018168, author = {浪越, 通夫 and Kapojos, Magie M. and 鵜飼, 和代 and 山﨑, 寛之 and Namikoshi, Michio and Ukai, Kazuyo and Yamazaki, Hiroyuki}, journal = {名古屋大学加速器質量分析計業績報告書}, month = {Mar}, note = {Di(2-ethylhexyl)phthalate (DEHP) was isolated from a culture broth of Streptomyces bangladeshensis strain NBRC 102113 and cultured cells of Amphidinium sp. to investigate if the isolated DEHP was a natural product. These microorganisms have been reported to provide DEHP, which may be produced by these microorganisms. The 14C contents (pMC) of DEHP and a positive control (a mixture of two fatty acids) purified from the ethanol extract of the dinoflagellate cells were 16.3 土 0.1 and 85.7 土 0.3, respectively, and DEHP and a mixture of two unsaturated fatty acids from the broth filtrate of S. bangladeshensis were 14.3 士 0.1 and 101.9 士 0.4, respectively. Consequently, the origin of DEHP isolated from these microorganisms was an industrial product. There were several reports that claimed DEHP was a microbial origin by the fermentation with radio active and stable isotope-labeled biosynthetic precursors. However, a labeled DEHP was not isolated but detected by HPLC or MS in these reports. Our study suggests at a labeled DEHP has to be purified and examined incorporated pattern to prove its origin.放線菌Streptomyces bangladeshensis., NBRC 102113株の培養液および渦鞭毛藻Amphidinium sp.培養藻体抽出物から単離したdi(2-ethylhexyl)phthalate (DEHP)が、これらの微生物によって生合成されている天然物であるかを検討した。それぞれ、培養液と培養藻体抽出画分からDEHPおよびコントロールとして脂肪酸をHPLCおよびGC分取により単離、精製した。これらDEHPと脂肪酸の14C濃度測定結果(pMC)はそれぞれ14.3土0.1と101.9土0.4(放線菌)および16.3土0.1と85.7土0.3(渦報毛藻)であった。この結果から、これらの放線菌培養液および渦鞭毛藻培養藻体から単離されたDEHPは工業製品であったと考えられる。, タンデトロン加速器質量分析計業績報告}, pages = {211--217}, title = {放線菌Streptomyces bangladeshensisの培養液および渦鞭毛藻Amphidinium sp.の培養藻体から単離したジ(2-エチルへキシル)フタレート(DEHP)の14C濃度測定結果について}, volume = {24}, year = {2013} }