{"created":"2021-05-31T02:40:30.075359+00:00","id":2000205,"links":{},"metadata":{"_buckets":{"deposit":"cf570690-c2a0-480c-8b8f-83443a8b1178"},"_deposit":{"created_by":17,"id":"2000205","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"2000205"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02000205","sets":["499:508:509:1622427158333"]},"author_link":[],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"86","bibliographicPageStart":"75","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Myh9 disorders are characterized by giant platelets, thrombocytopenia, and Döhle body-like cytoplasmic inclusion bodies in granulocytes. However, whether these disorders cause any changes in erythroid cells has yet to be determined. This study analyzed the influence of Myh9 R702C, as one of the most commonly detected Myh9 disorders, on erythroid cells in a mouse model.\nKnock-in mice expressing Myh9 R702C mutation either systemically or specific to hematological cells (R702C and R702C vav1 mice, respectively) were used in this study. Both displayed lower hemoglobin and higher erythropoietin levels than wild-type (WT) mice, along with significant splenomegaly. Flow cytometric analysis revealed erythroblasts present at a higher rate than WT mice in the spleen. However, no obvious abnormalities were seen in erythroid differentiation from megakaryocyte/erythroid progenitor to erythrocyte. Cell culture assay by fetal liver and colony assay also showed normal progression of erythroid differentiation from erythroid burst-forming unit to red blood cell.\nIn conclusion, R702C and R702C vav1 mice displayed erythroid abnormality with splenomegaly. However, erythroid differentiation showed no obvious abnormality. Further research is required to elucidate the underlying mechanisms.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.83.1.75","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University Graduate School of Medicine, School of Medicine","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/831.html","subitem_relation_type_select":"URI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kanematsu, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tamura, Shogo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Atsuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Katsumi, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kiyoi, Hitoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Hidehiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kunishima, Shinji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kojima, Tetsuhito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsushita, Tadashi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-05-31"}],"displaytype":"detail","filename":"07_Kanematsu.pdf","filesize":[{"value":"9.4 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"07_Kanematsu.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2000205/files/07_Kanematsu.pdf"},"version_id":"a1c899ec-4dde-43be-b472-da19dab40402"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MYH9 disorders","subitem_subject_scheme":"Other"},{"subitem_subject":"anemia","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse model","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Myh9 R702C is associated with erythroid abnormality with splenomegaly in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Myh9 R702C is associated with erythroid abnormality with splenomegaly in mice","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["1622427158333"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-05-31"},"publish_date":"2021-05-31","publish_status":"0","recid":"2000205","relation_version_is_last":true,"title":["Myh9 R702C is associated with erythroid abnormality with splenomegaly in mice"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T02:03:47.423304+00:00"}