{"created":"2021-11-11T01:51:14.518971+00:00","id":2001587,"links":{},"metadata":{"_buckets":{"deposit":"73575011-8e75-4081-8dfb-da8e57f61a2e"},"_deposit":{"created_by":17,"id":"2001587","owner":"17","owners":[17],"owners_ext":{"displayname":"図書情報係","username":"repository"},"pid":{"revision_id":0,"type":"depid","value":"2001587"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02001587","sets":["336:695:696"]},"author_link":[],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-11-11","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"316","bibliographicPageStart":"310","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Nature Plants","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The nitrate transporter NRT2.1, which plays a central role in high-affinity nitrate uptake in roots, is activated at the post-translational level in response to nitrogen (N) starvation. However, the critical enzymes required for the post-translational activation of NRT2.1 remain to be identified. Here, we show that a type 2C protein phosphatase, designated CEPD-induced phosphatase (CEPH), activates high-affinity nitrate uptake by directly dephosphorylating Ser501 of NRT2.1, a residue that functions as a negative phospho-switch in Arabidopsis. CEPH is predominantly expressed in epidermal and cortex cells in roots and is upregulated by N starvation via a CEPDL2/CEPD1/2-mediated long-distance signalling from shoots. The loss of CEPH leads to marked decreases in high-affinity nitrate uptake, tissue nitrate content and plant biomass. Collectively, our results identify CEPH as a crucial enzyme in the N-starvation-dependent activation of NRT2.1 and provide molecular and mechanistic insights into how plants regulate high-affinity nitrate uptake at the post-translational level in response to the N environment.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"nature","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41477-021-00870-9","subitem_relation_type_select":"DOI"}}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"2055-0278","subitem_source_identifier_type":"EISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohkubo, Yuri","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kuwata, Keiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsubayashi, Yoshikatsu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-11-11"}],"displaytype":"detail","filename":"CEPH_manuscript.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2001587/files/CEPH_manuscript.pdf"},"version_id":"997caab5-e956-4ec5-a013-c7211eca5bbf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["696"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-11"},"publish_date":"2021-11-11","publish_status":"0","recid":"2001587","relation_version_is_last":true,"title":["A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-01-16T05:02:54.137733+00:00"}