@article{oai:nagoya.repo.nii.ac.jp:02002163,
 author = {Nakagawa, Yu and Kakihara, Shintaro and Tsuzuki, Kazue and Ojika, Makoto and Igarashi, Yasuhiro and Ito, Yukishige},
 issue = {9},
 journal = {Journal of Natural Products},
 month = {Sep},
 note = {Pradimicin A (PRM-A) and related compounds constitute an exceptional family of natural pigments that show Ca^2+-dependent recognition of d-mannose (Man). Although these compounds hold great promise as research tools in glycobiology, their practical application has been severely limited by their inherent tendency to form water-insoluble aggregates. Here, we demonstrate that the 2-hydroxyethylamide derivative (PRM-EA) of PRM-A shows little aggregation in neutral aqueous media and retains binding specificity for Man. We also show that PRM-EA stains glycoproteins in dot blot assays, whereas PRM-A fails to do so, owing to severe aggregation. Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. Such staining selectivity has never been observed in conventional dyes, suggesting that PRM-EA could serve as a unique staining agent for the selective detection of glycoproteins with terminal Man residues.},
 pages = {2496--2501},
 title = {A Pradimicin-Based Staining Dye for Glycoprotein Detection},
 volume = {84},
 year = {2021}
}