{"created":"2022-03-08T05:47:03.889805+00:00","id":2002163,"links":{},"metadata":{"_buckets":{"deposit":"75408399-80ea-4134-86cc-c746a49ce682"},"_deposit":{"created_by":17,"id":"2002163","owner":"17","owners":[17],"owners_ext":{"displayname":"図書情報係","username":"repository"},"pid":{"revision_id":0,"type":"depid","value":"2002163"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02002163","sets":["643:666:667"]},"author_link":[],"control_number":"2002163","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-09-24","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"2501","bibliographicPageStart":"2496","bibliographicVolumeNumber":"84","bibliographic_titles":[{"bibliographic_title":"Journal of Natural Products","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Pradimicin A (PRM-A) and related compounds constitute an exceptional family of natural pigments that show Ca^2+-dependent recognition of d-mannose (Man). Although these compounds hold great promise as research tools in glycobiology, their practical application has been severely limited by their inherent tendency to form water-insoluble aggregates. Here, we demonstrate that the 2-hydroxyethylamide derivative (PRM-EA) of PRM-A shows little aggregation in neutral aqueous media and retains binding specificity for Man. We also show that PRM-EA stains glycoproteins in dot blot assays, whereas PRM-A fails to do so, owing to severe aggregation. Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. Such staining selectivity has never been observed in conventional dyes, suggesting that PRM-EA could serve as a unique staining agent for the selective detection of glycoproteins with terminal Man residues.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acs.jnatprod.1c00506","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Natural Products], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-A6KFIPPCDMPP5SRZ7UST].”","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0163-3864","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakagawa, Yu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kakihara, Shintaro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tsuzuki, Kazue","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ojika, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Igarashi, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ito, Yukishige","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-24"}],"displaytype":"detail","filename":"JNP2021_agr_nakagawa.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2002163/files/JNP2021_agr_nakagawa.pdf"},"version_id":"9727392a-7c85-420f-981c-53211c137eae"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Pradimicin-Based Staining Dye for Glycoprotein Detection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Pradimicin-Based Staining Dye for Glycoprotein Detection","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-08"},"publish_date":"2022-03-08","publish_status":"0","recid":"2002163","relation_version_is_last":true,"title":["A Pradimicin-Based Staining Dye for Glycoprotein Detection"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-01-16T04:42:40.894074+00:00"}