{"created":"2022-06-15T01:06:28.453260+00:00","id":2002961,"links":{},"metadata":{"_buckets":{"deposit":"80d45f97-90b8-4898-a6f3-38b6ea38ad88"},"_deposit":{"created_by":17,"id":"2002961","owner":"17","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"2002961"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02002961","sets":["499:500:501"]},"author_link":[],"control_number":"2002961","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-06-15","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1327","bibliographicPageStart":"1317","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"The American Journal of Sports Medicine","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Background: Wnt/β-catenin signaling suppresses the differentiation of cultured tenocytes, but its roles in tendon repair remain mostly elusive. No chemical compounds are currently available to treat tendon injury. Hypothesis: We hypothesized that the inhibition of Wnt/β-catenin signaling would accelerate tendon healing. Study Design: Controlled laboratory study. Methods: Tendon-derived cells (TDCs) were isolated from rat Achilles tendons. The right Achilles tendon was injured via a dermal punch, while the left tendon was sham operated. A Wnt/β-catenin inhibitor, IWR-1, and an antihistamine agent, promethazine (PH), were locally and intramuscularly injected, respectively, for 2 weeks after surgery. The healing tendons were histologically and biomechanically evaluated. Results: The amount of β-catenin protein was increased in the injured tendons from postoperative weeks 0.5 to 2. Inhibition of Wnt/β-catenin signaling by IWR-1 in healing tendons improved the histological abnormalities and decreased β-catenin, but it compromised the biomechanical properties. As we previously reported that antihistamine agents suppressed Wnt/β-catenin signaling in human chondrosarcoma cells, we examined the effects of antihistamines on TDCs. We found that a first-generation antihistamine agent, PH, increased the expression of the tendon marker genes Mkx and Tnmd in TDCs. Intramuscular injection of PH did not improve histological abnormalities, but it decreased β-catenin in healing tendons and increased the peak force and stiffness of the healing tendons on postoperative week 2. On postoperative week 8, however, the biomechanical properties of vehicle-treated tendons became similar to those of PH-treated tendons. Conclusion: IWR-1 and PH suppressed Wnt/β-catenin signaling and improved the histological abnormalities of healing tendons. IWR-1, however, compromised the biomechanical properties of healing tendons, whereas PH improved them.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SAGE Publications","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1177/03635465221077116","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Takefumi Sakaguchi, Bisei Ohkawara, Yasuzumi Kishimoto, Kentaro Miyamoto, Shinya Ishizuka, Hideki Hiraiwa, Naoki Ishiguro, Imagama, Shiro, Ohno, Kinji. Promethazine Downregulates Wnt/β-Catenin Signaling and Increases the Biomechanical Forces of the Injured Achilles Tendon in the Early Stage of Healing. The American Journal of Sports Medicine (v.50, n.5) pp.1317-1327. Copyright © [2022] (AOSSM). DOI: [10.1177/03635465221077116].","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0363-5465","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakaguchi, Takefumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohkawara, Bisei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kishimoto, Yasuzumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyamoto, Kentaro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishizuka, Shinya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hiraiwa, Hideki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishiguro, Naoki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Imagama, Shiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohno, Kinji","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-15"}],"displaytype":"detail","filename":"Manuscript_20211125bw.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2002961/files/Manuscript_20211125bw.pdf"},"version_id":"a709fb0f-43a1-4e22-854a-290f78e6ca65"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-15"}],"displaytype":"detail","filename":"Supplementary_information_20211125bw.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"dataset","url":"https://nagoya.repo.nii.ac.jp/record/2002961/files/Supplementary_information_20211125bw.pdf"},"version_id":"7a9a12cb-aef3-419e-bd63-360ef6556ee5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"promethazine","subitem_subject_scheme":"Other"},{"subitem_subject":"Achilles tendon","subitem_subject_scheme":"Other"},{"subitem_subject":"tendon healing","subitem_subject_scheme":"Other"},{"subitem_subject":"drug repositioning","subitem_subject_scheme":"Other"},{"subitem_subject":"biomechanics of the tendon","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Promethazine Downregulates Wnt/β-Catenin Signaling and Increases the Biomechanical Forces of the Injured Achilles Tendon in the Early Stage of Healing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Promethazine Downregulates Wnt/β-Catenin Signaling and Increases the Biomechanical Forces of the Injured Achilles Tendon in the Early Stage of Healing","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-15"},"publish_date":"2022-06-15","publish_status":"0","recid":"2002961","relation_version_is_last":true,"title":["Promethazine Downregulates Wnt/β-Catenin Signaling and Increases the Biomechanical Forces of the Injured Achilles Tendon in the Early Stage of Healing"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-01-16T04:44:51.779730+00:00"}