{"_buckets": {"deposit": "5b590034-be7b-4f08-acf3-fe22f3f2138d"}, "_deposit": {"created_by": 17, "id": "2003778", "owner": "17", "owners": [17], "owners_ext": {"displayname": "repository", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2003778"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02003778", "sets": ["667"]}, "author_link": [], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "日付", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2023-06-01", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "651", "bibliographicPageStart": "641", "bibliographicVolumeNumber": "171", "bibliographic_titles": [{"bibliographic_title": "The Journal of Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "cis-Prenyltransferases (cPTs) form linear polyprenyl pyrophosphates, the precursors of polyprenyl or dolichyl phosphates that are essential for cell function in all living organisms. Polyprenyl phosphate serves as a sugar carrier for peptidoglycan cell wall synthesis in bacteria, a role that dolichyl phosphate performs analogously for protein glycosylation in eukaryotes and archaea. Bacterial cPTs are characterized by their homodimeric structure, while cPTs from eukaryotes usually require two distantly homologous subunits for enzymatic activity. This study identifies the subunits of heteromeric cPT, Af1219 and Af0707, from a thermophilic sulphur-reducing archaeon, Archaeoglobus fulgidus. Both subunits are indispensable for cPT activity, and their protein–protein interactions were demonstrated by a pulldown assay. Gel filtration chromatography and chemical cross-linking experiments suggest that Af1219 and Af0707 likely form a heterotetramer complex. Although this expected subunit composition agrees with a reported heterotetrameric structure of human hCIT/NgBR cPT complex, the similarity of the quaternary structures is likely a result of convergent evolution.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/jb/mvac022", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is a pre-copyedited, author-produced version of an article accepted for publication in [The Journal of Biochemistry] following peer review. The version of record [Kitty Sompiyachoke, Arisa Nagasaka, Tomokazu Ito, Hisashi Hemmi, Identification and biochemical characterization of a heteromeric cis-prenyltransferase from the thermophilic archaeon Archaeoglobus fulgidus, The Journal of Biochemistry, Volume 171, Issue 6, June 2022, Pages 641–651, https://doi.org/10.1093/jb/mvac022] is available online at: https://doi.org/10.1093/jb/mvac022.", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0021-924X", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sompiyachoke, Kitty", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagasaka, Arisa", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ito, Tomokazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hemmi, Hisashi", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-06-01"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 2023/5/31.", "file_order": 0, "filename": "manuscript_forrepository.pdf", "filesize": [{"value": "3.7 MB"}], "format": "application/pdf", "future_date_message": "Download is available from 2023/5/31.", "is_thumbnail": false, "mimetype": "application/pdf", "size": 3700000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2003778/files/manuscript_forrepository.pdf"}, "version_id": "ecab62e0-558b-471a-9da1-264fd12f35df"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "archaea", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cis-prenyl transferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "dolichol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "enzyme structure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "isoprenoid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and biochemical characterization of a heteromeric cis-prenyltransferase from the thermophilic archaeon Archaeoglobus fulgidus", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Identification and biochemical characterization of a heteromeric cis-prenyltransferase from the thermophilic archaeon Archaeoglobus fulgidus", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "17", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/0002003778", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-09-14"}, "publish_date": "2022-09-14", "publish_status": "0", "recid": "2003778", "relation": {}, "relation_version_is_last": true, "title": ["Identification and biochemical characterization of a heteromeric cis-prenyltransferase from the thermophilic archaeon Archaeoglobus fulgidus"], "weko_shared_id": -1}