{"created":"2022-09-15T05:49:37.664618+00:00","id":2003794,"links":{},"metadata":{"_buckets":{"deposit":"b14fa130-29e8-4ed7-8f05-20c53ca3ce21"},"_deposit":{"created_by":17,"id":"2003794","owner":"17","owners":[17],"owners_ext":{"displayname":"repository","username":"repository"},"pid":{"revision_id":0,"type":"depid","value":"2003794"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02003794","sets":["320:321:322"]},"author_link":[],"control_number":"2003794","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-04-06","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"5892","bibliographicPageStart":"5887","bibliographicVolumeNumber":"144","bibliographic_titles":[{"bibliographic_title":"Journal of the American Chemical Society","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Construction of complex DNA circuits is difficult due to unintended hybridization and degradation by enzymes under biological conditions. We herein report a hybridization chain reaction (HCR) circuit composed of left-handed acyclicd-threoninol nucleic acid (d-aTNA), which is orthogonal to right-handed DNA and RNA. Because of its high thermal stability, use of an aTNA hairpin with a short 7 base-pair stem ensured clear ON–OFF control of the HCR circuit. The aTNA circuit was stable against nucleases. A circuit based on right-handed acyclicl-threoninol nucleic acid (l-aTNA) was also designed, and high orthogonality between d- and l-aTNA HCRs was confirmed by activation of each aTNA HCR via a corresponding input strand. A dual OR logic gate was successfully established using serinol nucleic acid (SNA), which could initiate both d- and l-aTNA circuits. The d-aTNA HCR was used for an RNA-dependent signal amplification system via the SNA interface. The design resulted in 80% yield of the cascade reaction in 3000 s without a significant leak. This work represents the first example of use of heterochiral HCR circuits for detection of RNA molecules. The method has potential for direct visualization of RNA in vivo and the FISH method.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/jacs.1c12659","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of the American Chemical Society], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-RH5U3UUWSXYEGFECEVGM].”","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0002-7863","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chen, Yanglingzhi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagao, Ryuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Murayama, Keiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Asanuma, Hiroyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-06"}],"displaytype":"detail","filename":"Manuscript-XNA-HCR-rev2.pdf","filesize":[{"value":"968 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2003794/files/Manuscript-XNA-HCR-rev2.pdf"},"version_id":"e94db65c-3a57-46aa-8513-cae5a3ad09c5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Orthogonal Amplification Circuits Composed of Acyclic Nucleic Acids Enable RNA Detection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Orthogonal Amplification Circuits Composed of Acyclic Nucleic Acids Enable RNA Detection","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["322"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-15"},"publish_date":"2022-09-15","publish_status":"0","recid":"2003794","relation_version_is_last":true,"title":["Orthogonal Amplification Circuits Composed of Acyclic Nucleic Acids Enable RNA Detection"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-05-08T00:41:42.945692+00:00"}