{"_buckets": {"deposit": "ec5101f0-cc42-4303-a50d-6a3108ea8b33"}, "_deposit": {"created_by": 17, "id": "2003920", "owner": "17", "owners": [17], "owners_ext": {"displayname": "repository", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2003920"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02003920", "sets": ["2034"]}, "author_link": [], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "日付", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2023-08-20", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-08-20", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "21", "bibliographicPageStart": "16", "bibliographicVolumeNumber": "617", "bibliographic_titles": [{"bibliographic_title": "Biochemical and Biophysical Research Communications", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "The CMP-sialic acid synthetase (CSS) activates free sialic acid (Sia) to CMP-Sia using CTP, and is prerequisite for the sialylation of cell surface glycoconjugates. The vertebrate CSS consists of two domains, a catalytic N-domain and a non-catalytic C-domain. Although the C-domain is not required for the CSS enzyme to synthesize CMP-Sia, its involvement in the catalytic activity remains unknown. First, the real-time monitoring of CSS-catalyzed reaction was performed by 31P NMR using the rainbow trout CSS (rtCSS). While a rtCSS lacking the C-domain (rtCSS-N) similarly activated both deaminoneuraminic acid (Kdn) and N-acetylneuraminic acid (Neu5Ac), the full-length rtCSS (rtCSS-FL) did not activate Kdn as efficiently as Neu5Ac. These results suggest that the C-domain of rtCSS affects the enzymatic activity, when Kdn was used as a substrate. Second, the enzymatic activity of rtCSS-FL and rtCSS-N was measured under various concentrations of CMP-Kdn. Inhibition by CMP-Kdn was observed only for rtCSS-FL, but not for rtCSS-N, suggesting that the inhibition was C-domain-dependent. Third, the inhibitory effect of CMP-Kdn was also investigated using the mouse CSS (mCSS). However, no inhibition was observed with mCSS even at high concentrations of CMP-Kdn. Taken together, the data demonstrated that the C-domain is involved in the CMP-Kdn-dependent inhibition of rtCSS, which is a novel regulation of the Sia metabolism in rainbow trout.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbrc.2022.05.031", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Wu, Di", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Gilormini, Pierre-André", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toda, Sakura", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Biot, Christophe", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Lion, Cédric", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Guérardel, Yann", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sato, Chihiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kitajima, Ken", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-08-20"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 2023/8/19.", "file_order": 0, "filename": "WD_BBRC_617_2022.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "Download is available from 2023/8/19.", "is_thumbnail": false, "mimetype": "application/pdf", "size": 1200000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2003920/files/WD_BBRC_617_2022.pdf"}, "version_id": "fef98daf-484d-4682-ab40-84f520cafaf1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "CMAS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CSS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CMP-Sialic acid synthetase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Deaminoneuraminic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "N-acetylneuraminic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Rainbow trout", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sialic acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "17", "path": ["2034"], "permalink_uri": "http://hdl.handle.net/2237/0002003920", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-10-13"}, "publish_date": "2022-10-13", "publish_status": "0", "recid": "2003920", "relation": {}, "relation_version_is_last": true, "title": ["A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid"], "weko_shared_id": -1}