{"created":"2022-10-13T04:42:41.433881+00:00","id":2003920,"links":{},"metadata":{"_buckets":{"deposit":"9b71b51f-1988-472c-95d5-18f4c740eb04"},"_deposit":{"created_by":17,"id":"2003920","owner":"17","owners":[17],"owners_ext":{"displayname":"repository","username":"repository"},"pid":{"revision_id":0,"type":"depid","value":"2003920"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02003920","sets":["1179:2033:2034"]},"author_link":[],"control_number":"2003920","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-08-20","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-08-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21","bibliographicPageStart":"16","bibliographicVolumeNumber":"617","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The CMP-sialic acid synthetase (CSS) activates free sialic acid (Sia) to CMP-Sia using CTP, and is prerequisite for the sialylation of cell surface glycoconjugates. The vertebrate CSS consists of two domains, a catalytic N-domain and a non-catalytic C-domain. Although the C-domain is not required for the CSS enzyme to synthesize CMP-Sia, its involvement in the catalytic activity remains unknown. First, the real-time monitoring of CSS-catalyzed reaction was performed by 31P NMR using the rainbow trout CSS (rtCSS). While a rtCSS lacking the C-domain (rtCSS-N) similarly activated both deaminoneuraminic acid (Kdn) and N-acetylneuraminic acid (Neu5Ac), the full-length rtCSS (rtCSS-FL) did not activate Kdn as efficiently as Neu5Ac. These results suggest that the C-domain of rtCSS affects the enzymatic activity, when Kdn was used as a substrate. Second, the enzymatic activity of rtCSS-FL and rtCSS-N was measured under various concentrations of CMP-Kdn. Inhibition by CMP-Kdn was observed only for rtCSS-FL, but not for rtCSS-N, suggesting that the inhibition was C-domain-dependent. Third, the inhibitory effect of CMP-Kdn was also investigated using the mouse CSS (mCSS). However, no inhibition was observed with mCSS even at high concentrations of CMP-Kdn. Taken together, the data demonstrated that the C-domain is involved in the CMP-Kdn-dependent inhibition of rtCSS, which is a novel regulation of the Sia metabolism in rainbow trout.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2022.05.031","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wu, Di","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Gilormini, Pierre-André","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Toda, Sakura","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Biot, Christophe","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Lion, Cédric","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Guérardel, Yann","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sato, Chihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kitajima, Ken","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-08-20"}],"displaytype":"detail","filename":"WD_BBRC_617_2022.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2003920/files/WD_BBRC_617_2022.pdf"},"version_id":"8c94f683-225a-44d5-9988-fc300f6f5e9e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CMAS","subitem_subject_scheme":"Other"},{"subitem_subject":"CSS","subitem_subject_scheme":"Other"},{"subitem_subject":"CMP-Sialic acid synthetase","subitem_subject_scheme":"Other"},{"subitem_subject":"Deaminoneuraminic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"N-acetylneuraminic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Rainbow trout","subitem_subject_scheme":"Other"},{"subitem_subject":"Sialic acid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["2034"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-10-13"},"publish_date":"2022-10-13","publish_status":"0","recid":"2003920","relation_version_is_last":true,"title":["A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-09-08T01:54:16.325940+00:00"}