{"_buckets": {"deposit": "60814c4d-d21b-4d96-91db-6df00542f09b"}, "_deposit": {"created_by": 17, "id": "2004228", "owner": "17", "owners": [17], "owners_ext": {"displayname": "repository", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2004228"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02004228", "sets": ["2034"]}, "author_link": [], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "日付", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2023-09-01", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "701", "bibliographicPageStart": "693", "bibliographicVolumeNumber": "135", "bibliographic_titles": [{"bibliographic_title": "Journal of Plant Research", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Plasmodesmata are unique channel structures in plants that link the fluid cytoplasm between adjacent cells. Plants have evolved these microchannels to allow trafficking of nutritious substances as well as regulatory factors for intercellular communication. However, tracking the behavior of plasmodesmata in real time is difficult because they are located inside tissues. Hence, a system was constructed to monitor the movement of substances by plasmodesmata using tobacco BY-2 cells, which are linearly organized cells, and a microfluidic device that traps them in place and facilitates observation. After targeting one cell for photobleaching, recovery of the lost H2B-GFP protein was detected within 200 min. No recovery was detected in that time frame by photobleaching the entire cell filaments. This suggested that the recovery of H2B-GFP protein was not due to de novo protein synthesis, but rather to translocation from neighboring cells. The transport of H2B-GFP protein was not observed when sodium chloride, a compound known to cause plasmodesmata closure, was present in the microfluid channel. Thus, using the microfluidic device and BY-2 cells, it was confirmed that the behavior of plasmodesmata could be observed in real time under controllable conditions.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Nature", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s10265-022-01406-8", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10265-022-01406-8", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0918-9440", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kurotani, Ken-ichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kawakatsu, Yaichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kikkawa, Masahiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tabata, Ryo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kurihara, Daisuke", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Honda, Hiroyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimizu, Kazunori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Notaguchi, Michitaka", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-09-01"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 2023/8/31.", "file_order": 0, "filename": "openaccess_Kurotani_et_al.pdf", "filesize": [{"value": "3.9 MB"}], "format": "application/pdf", "future_date_message": "Download is available from 2023/8/31.", "is_thumbnail": false, "mimetype": "application/pdf", "size": 3900000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2004228/files/openaccess_Kurotani_et_al.pdf"}, "version_id": "3687569e-00b2-4f18-96f9-2d72580e9dfe"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Analysis of plasmodesmata permeability using cultured tobacco BY-2 cells entrapped in microfluidic chips", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Analysis of plasmodesmata permeability using cultured tobacco BY-2 cells entrapped in microfluidic chips", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "17", "path": ["2034"], "permalink_uri": "http://hdl.handle.net/2237/0002004228", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-11-29"}, "publish_date": "2022-11-29", "publish_status": "0", "recid": "2004228", "relation": {}, "relation_version_is_last": true, "title": ["Analysis of plasmodesmata permeability using cultured tobacco BY-2 cells entrapped in microfluidic chips"], "weko_shared_id": -1}