{"_buckets": {"deposit": "37f845f7-c1dc-4425-8d71-a7a94311744b"}, "_deposit": {"created_by": 17, "id": "2004245", "owner": "17", "owners": [17], "owners_ext": {"displayname": "repository", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2004245"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02004245", "sets": ["322"]}, "author_link": [], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "日付", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2023-11-01", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "3318", "bibliographicPageStart": "3311", "bibliographicVolumeNumber": "119", "bibliographic_titles": [{"bibliographic_title": "Biotechnology and Bioengineering", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Endochondral ossification is the process of bone formation derived from growing cartilage duringskeletal development. In previous studies, we provoked the osteocyte differentiation of osteoblast precursor cells under a three-dimensional (3D) culture model. To recapitulate the endochondral ossification, the present study utilized the self-organized scaffold-free spheroid model reconstructed by pre-chondrocyte cells. Within 2-day cultivation in the absence of the chemically induced chondrogenesis supplements, the chondrocyte marker was greatly expressed in the inner region of the spheroid, whereas the hypertrophic chondrocyte marker was strongly detected in the surface region of the spheroid. Notably, we found out that the gene expression levels of osteocyte markers were also greatly upregulated compared to the conventional 2D monolayer. Moreover, after long-term cultivation for 28 days, it induced morphological changes in the spheroid, such as cellular hypertrophy and death. In this study, in order to recapitulate the initial stage of the endochondral ossification, we highlighted the potentials of the 3D culture method to drive the hypertrophic chondrocyte differentiation of the pre-chondrocyte cells.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/bit.28203", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [ Kim, J., Tomida, K., Matsumoto, T., \u0026 Adachi, T. (2022). Spheroid culture for chondrocytes triggers the initial stage of endochondral ossification. Biotechnology and Bioengineering, 119, 3311– 3318. https://doi.org/10.1002/bit.28203], which has been published in final form at [https://doi.org/10.1002/bit.28203]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.\"", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0006-3592", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kim, Jeonghyun", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tomida, Kosei", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Takeo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Adachi, Taiji", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-11-01"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 2023/10/31.", "file_order": 0, "filename": "Manuscript_final.pdf", "filesize": [{"value": "302 KB"}], "format": "application/pdf", "future_date_message": "Download is available from 2023/10/31.", "is_thumbnail": false, "mimetype": "application/pdf", "size": 302000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2004245/files/Manuscript_final.pdf"}, "version_id": "07115ee6-a959-4392-ac3f-862c429019e7"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-11-01"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 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