{"_buckets": {"deposit": "bc7e2c62-947a-4e46-9f1f-ab29b6e909d7"}, "_deposit": {"id": "2005197", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2005197"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02005197", "sets": ["1679636128616"]}, "author_link": [], "control_number": "2005197", "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-03-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "11", "bibliographicPageStart": "7", "bibliographicVolumeNumber": "25", "bibliographic_titles": [{"bibliographic_title": "総合保健体育科学", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Nagoya Journal of Health, Physical Fitness \u0026 Sports", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "The α-ketoisocaproate dioxygenase (KICD) catalyzes the decarboxylation of α-ketoisocaproate (KIC). derived from transamination of leucine, to β-hydroxy-β-methylbutyrate (HMB) in the cytosol of the liver. The characterization of the purified KICD from rat and human livers indicated that this enzyme requires Fe^2+ for its activity, but does not use CoA-SH and NAD^+ as cofactors that are required for the branched-chain α-keto acid dehydrogenase complex (BCKDC), which catalyzes the decarboxylation of KIC to isovalery-CoA in mitochondria. In the present study, a radiochemical assay was developed to measure the KICD activity in liver extracts. In this method, the decarboxylation of KIC by BCKDC was eliminated by addition of BCKDC-antiserum in the assay mixture. Evidence is provided here for establishment of improved assay conditions for KICD in liver extracts.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/njhpfs.25.1.7", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "名古屋大学総合保健体育科学センター", "subitem_publisher_language": "ja"}, {"subitem_publisher": "The Research Center of Health, Physical Fitness and Sports, Nagoya University", "subitem_publisher_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0289-5412", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Xu, Ming", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagasaki, Masaru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Li, Zhihao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Obayashi, Mariko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Baggiotto, Gustavo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sato, Yuzo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimomura, Yoshiharu", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2023-03-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "njhpfs_25_1_7.pdf", "filesize": [{"value": "3.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 3100000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2005197/files/njhpfs_25_1_7.pdf"}, "version_id": "d77a8699-8f84-4856-b307-1c57a5f32139"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "1", "path": ["1679636128616"], "permalink_uri": "https://doi.org/10.18999/njhpfs.25.1.7", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2023-03-27"}, "publish_date": "2023-03-27", "publish_status": "0", "recid": "2005197", "relation": {}, "relation_version_is_last": true, "title": ["Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers"], "weko_shared_id": -1}