{"created":"2023-03-24T05:36:47.953224+00:00","id":2005197,"links":{},"metadata":{"_buckets":{"deposit":"bc7e2c62-947a-4e46-9f1f-ab29b6e909d7"},"_deposit":{"id":"2005197","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005197"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02005197","sets":["1213:1620:1621:1679636128616"]},"author_link":[],"control_number":"2005197","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"11","bibliographicPageStart":"7","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"総合保健体育科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"Nagoya Journal of Health, Physical Fitness & Sports","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The α-ketoisocaproate dioxygenase (KICD) catalyzes the decarboxylation of α-ketoisocaproate (KIC). derived from transamination of leucine, to β-hydroxy-β-methylbutyrate (HMB) in the cytosol of the liver. The characterization of the purified KICD from rat and human livers indicated that this enzyme requires Fe^2+ for its activity, but does not use CoA-SH and NAD^+ as cofactors that are required for the branched-chain α-keto acid dehydrogenase complex (BCKDC), which catalyzes the decarboxylation of KIC to isovalery-CoA in mitochondria. In the present study, a radiochemical assay was developed to measure the KICD activity in liver extracts. In this method, the decarboxylation of KIC by BCKDC was eliminated by addition of BCKDC-antiserum in the assay mixture. Evidence is provided here for establishment of improved assay conditions for KICD in liver extracts.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/njhpfs.25.1.7","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋大学総合保健体育科学センター","subitem_publisher_language":"ja"},{"subitem_publisher":"The Research Center of Health, Physical Fitness and Sports, Nagoya University","subitem_publisher_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0289-5412","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Xu, Ming","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nagasaki, Masaru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Li, Zhihao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Obayashi, Mariko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Baggiotto, Gustavo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sato, Yuzo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimomura, Yoshiharu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-27"}],"displaytype":"detail","filename":"njhpfs_25_1_7.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2005197/files/njhpfs_25_1_7.pdf"},"version_id":"d77a8699-8f84-4856-b307-1c57a5f32139"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"1","path":["1679636128616"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-27"},"publish_date":"2023-03-27","publish_status":"0","recid":"2005197","relation_version_is_last":true,"title":["Determination of α-Ketoisocaproate Dioxygenase Activity in Mammalian Livers"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-03-27T02:07:51.560948+00:00"}