@article{oai:nagoya.repo.nii.ac.jp:02005582, author = {楊, 衛平 and YANG, Wei-Ping and 呉, 瑋 and WU, Wei and 押田, 芳治 and OSHIDA, Yoshiharu and 大沢, 功 and OHSAWA, Isao and 佐藤, 寿一 and SATO, Juichi and 佐藤, 祐造 and SATO, Yuzo}, issue = {1}, journal = {総合保健体育科学, Nagoya Journal of Health, Physical Fitness & Sports}, month = {Mar}, note = {In order to elucidate the effects of skeletal muscle contraction and insulin on glucose uptake in exercised muscle, we studied 19 male Wistar rats by using microdialysis technique in both femoral muscles and jugular vein. Under the continuous somatostatin infusion (0.5 μg/kg·min) and bolus infusion of anti-insulin serum (0.3ml), which induced a complete suppresson of insulin secretion (plasma insulin levels were less than 3μU/ml), these rats were divided into 2 groups. One was that both femoral muscles were stimulated electrically (S). The other was that both thighs were kept resting without stimulation (C). The lactate concentration in dialysate were determined every 15minutes and the dialysate lactate concentration in muscle was a measure of glucose uptake. After somatostatin infusion (2 hours), insulin was infused continuously(6.0mU/kg·min) in both groups. The following results were obtained: 1. In S group., the dialysate lactate concentrations in muscle were significantly higher than that in jugular vein under the condition of complete suppression of insulin secretion. 2. In C group, the dialysate lactate levels in muscle were significantly lower than that in jugular vein under the above condition. 3. During inslin infusion, the increase in dialysate lactate concentration in muscle of S group was almost similar to that of C group. From these results, it could be confirmed that exercised muscle results in a large increase in glucose uptake even if insulin secretion is suppressed completely, and that insulin acts as an additive effect in exercised muscle.}, pages = {23--25}, title = {筋収縮とインスリンが骨格筋のglycolysisに及ぼす影響について : Microdialysis法による検討}, volume = {15}, year = {1992} }