{"created":"2023-10-18T06:27:46.578858+00:00","id":2007900,"links":{},"metadata":{"_buckets":{"deposit":"dc6c0659-9ebb-4cbd-92f5-d915e380847c"},"_deposit":{"created_by":17,"id":"2007900","owner":"17","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"2007900"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02007900","sets":["499:500:501"]},"author_link":[],"control_number":"2007900","item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1629683748249":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2024-05-01","subitem_date_issued_type":"Available"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"154443","bibliographicVolumeNumber":"245","bibliographic_titles":[{"bibliographic_title":"Pathology - Research and Practice","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Osteosarcoma, the most common primary malignant bone tumor, is defined by the formation of neoplastic osteoid and/or bone. This sarcoma is a highly heterogeneous disease with a wide range of patient outcomes. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed in various types of malignant tumors. We previously reported that CD109 is expressed in osteoblasts and osteoclasts in normal human tissues and plays a role in bone metabolism in vivo. While CD109 has been shown to promote various carcinomas through the downregulation of TGF-β signaling, the role and mechanism of CD109 in sarcomas remain largely unknown. In this study, we investigated the molecular function of CD109 in sarcomas using osteosarcoma cell lines and tissue. Semi-quantitative immunohistochemical analysis using human osteosarcoma tissue revealed a significantly worse prognosis in the CD109-high group compared with the CD109-low group. We found no association between CD109 expression and TGF-β signaling in osteosarcoma cells. However, enhancement of SMAD1/5/9 phosphorylation was observed in CD109 knockdown cells under bone morphogenetic protein-2 (BMP-2) stimulation. We also performed immunohistochemical analysis for phospho-SMAD1/5/9 using human osteosarcoma tissue and found a negative correlation between CD109 expression and SMAD1/5/9 phosphorylation. In vitro wound healing assay showed that osteosarcoma cell migration was significantly attenuated in CD109-knockdown cells compared with control cells in the presence of BMP. These results suggest that CD109 is a poor prognostic factor in osteosarcoma and affects tumor cell migration via BMP signaling.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.prp.2023.154443","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0344-0338","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mori, Natsumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Esaki, Nobutoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimoyama, Yoshie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shiraki, Yukihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Asai, Naoya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sakai, Tomohisa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nishida, Yoshihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takahashi, Masahide","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Enomoto, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mii, Shinji","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-05-01"}],"displaytype":"detail","filename":"01_20230330_bone-tumor.pdf","filesize":[{"value":"65.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2007900/files/01_20230330_bone-tumor.pdf"},"version_id":"8c6eccce-bf8f-4349-a69a-b0d608a96958"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-05-01"}],"displaytype":"detail","filename":"02_20230328_bone-tumor_supple.pdf","filesize":[{"value":"32.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"dataset","url":"https://nagoya.repo.nii.ac.jp/record/2007900/files/02_20230328_bone-tumor_supple.pdf"},"version_id":"ea88be29-159c-4c98-ba41-cf505f8e2d11"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CD109","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteosarcoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Prognostic marker","subitem_subject_scheme":"Other"},{"subitem_subject":"BMP","subitem_subject_scheme":"Other"},{"subitem_subject":"SMAD","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-10-18"},"publish_date":"2023-10-18","publish_status":"0","recid":"2007900","relation_version_is_last":true,"title":["Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-05-13T00:16:06.222128+00:00"}